Active Recombinant Mouse Il2 Protein, His-Tagged

• Cat.No.: Il2-01M
• Product Overview: Recombinant mouse Il2 Protein, His-tagged was expressed in E. coli cell

Specification

• Description: Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes.
• Source: Escherichia coli
• Species: Mouse
• Tag: His
• Form: Lyophilized powder
• AA Sequence: MAPTSSSTSSSTAEAQQQQQQQQQQ QQHLEQLLMDLQELLSRMENYRNLK LPRMLTFKFYLPKQATELKDLQCLE DELGPLRHVLDLTQS
  KSFQLEDAENFISNIRVTVVKLKGS DNTFECQFDDESATVVDFLRRWIAF CQSIISTSPQ with polyhistidine tag at the C-terminus
• Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
• Bio-activity: Measure by its ability to induce CTLL-2 cells proliferation. The ED50 for this effect is <0.3 ng/mL. The specific activity of recombinant mouse IL-2 is approximately >3 x 10^6 IU/mg.
• Purity: ≥98% as determined by SDS-PAGE and HPLC.
  Purified by Ni-NTA chromatography.
• Storage Buffer: The protein was lyophilized from a solution containing 1X PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Storage: Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
• Notes: Please use within one month after protein reconstitution.

Gene Information

• Gene Name: Il2 interleukin 2 [ Mus musculus (house mouse) ]
• Official Symbol: Il2
• Synonyms: Il-2
• Gene ID: 16183
• mRNA Refseq: NM_008366.3
• Protein Refseq: NP_032392.1
• UniProt ID: P04351