Recombinant Mouse Lgmn Protein, Myc/DDK-tagged

• Cat.No.: Lgmn-3778M
• Product Overview: Purified recombinant protein of mouse full-length legumain (Lgmn), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes a member of the cysteine peptidase family C13 that plays an important role in the endosome/lysosomal degradation system. The encoded inactive preproprotein undergoes autocatalytic removal of the C-terminal inhibitory propeptide to generate the active endopeptidase that cleaves protein substrates on the C-terminal side of asparagine residues. Mice lacking the encoded protein exhibit defects in the lysosomal processing of proteins resulting in their accumulation in the lysosomes, and develop symptoms resembling hemophagocytic lymphohistiocytosis.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 49.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Lgmn legumain [ Mus musculus (house mouse) ]
• Official Symbol: Lgmn
• Synonyms: LGMN; legumain; preprolegumain; protease, cysteine 1; protease, cysteine, 1; asparaginyl endopeptidase; AEP; Prsc1; AI746452; AU022324
• Gene ID: 19141
• mRNA Refseq: NM_011175
• Protein Refseq: NP_035305
• UniProt ID: O89017