Recombinant Mouse Mdh1 Protein, Myc/DDK-tagged

• Cat.No.: Mdh1-4002M
• Product Overview: Purified recombinant protein of mouse full-length malate dehydrogenase 1, NAD (soluble) (Mdh1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. A pseudogene has been identified on chromosomes 12.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 36.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mdh1 malate dehydrogenase 1, NAD (soluble) [ Mus musculus (house mouse) ]
• Official Symbol: Mdh1
• Synonyms: MDH1; malate dehydrogenase 1, NAD (soluble); malate dehydrogenase, cytoplasmic; malate dehydrogenase, soluble; cytosolic malate dehydrogenase; MDHA; Mor2; MDH-s; Mor-2; D17921; B230377B03Rik
• Gene ID: 17449
• mRNA Refseq: NM_008618
• Protein Refseq: NP_032644
• UniProt ID: P14152