Recombinant Mouse Mettl1 Protein, Myc/DDK-tagged

• Cat.No.: Mettl1-4040M
• Product Overview: Purified recombinant protein of mouse full-length methyltransferase like 1 (Mettl1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Methyltransferase that mediates the formation of N7-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA. Also acts as a methyltransferase for a subset of internal N7-methylguanine in mRNAs. Internal N7-methylguanine methylation of mRNAs regulates translation. Also methylates a specific subset of miRNAs, such as let-7. N7-methylguanine methylation of let-7 miRNA promotes let-7 miRNA processing by disrupting an inhibitory secondary structure within the primary miRNA transcript (pri-miRNA). Acts as a regulator of embryonic stem cell self-renewal and differentiation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 30.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mettl1 methyltransferase like 1 [ Mus musculus (house mouse) ]
• Official Symbol: Mettl1
• Synonyms: METTL1; methyltransferase like 1; tRNA (guanine-N(7)-)-methyltransferase; methyltransferase-like 1; tRNA(m7G46)-methyltransferase; methyltransferase-like protein 1; tRNA (guanine(46)-N(7))-methyltransferase; 2810012D02Rik
• Gene ID: 17299
• mRNA Refseq: NM_010792
• Protein Refseq: NP_034922
• UniProt ID: Q9Z120