Recombinant Mouse Mink1 Protein, Myc/DDK-tagged

• Cat.No.: Mink1-4075M
• Product Overview: Purified recombinant protein of mouse full-length misshapen-like kinase 1 (zebrafish) (cDNA clone MGC:63403 IMAGE:6833893), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates: TANC1 upon stimulation by RAP2A, MBP and SMAD1. Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 150.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mink1 misshapen-like kinase 1 (zebrafish) [ Mus musculus (house mouse) ]
• Official Symbol: Mink1
• Synonyms: Mink1; misshapen-like kinase 1 (zebrafish); MI; B55; Ysk; MINK; Map4; Ysk2; Map4k6; misshapen-like kinase 1; GCK family kinase MiNK; MAPK/ERK kinase kinase kinase 6; MEK kinase kinase 6; MEKKK 6; Misshapen/NIKs-related kinase; Yeast Sps1/Ste20-related kinase 2; misshapen/NIK-related kinase; EC 2.7.11.1
• Gene ID: 50932
• mRNA Refseq: NM_016713
• Protein Refseq: NP_057922
• UniProt ID: Q9JM52