Recombinant Mouse Nmral1 Protein, Myc/DDK-tagged

• Cat.No.: Nmral1-4445M
• Product Overview: Purified recombinant protein of mouse full-length NmrA-like family domain containing 1 (Nmral1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Redox sensor protein. Undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP+ levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. The homodimer binds one molecule of NADPH. Has higher affinity for NADPH than for NADP+. Binding to NADPH is necessary to form a stable dimer.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 34.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nmral1 NmrA-like family domain containing 1 [ Mus musculus (house mouse) ]
• Official Symbol: Nmral1
• Synonyms: NMRAL1; NmrA-like family domain containing 1; nmrA-like family domain-containing protein 1; AI256624; 1110025F24Rik
• Gene ID: 67824
• mRNA Refseq: NM_026393
• Protein Refseq: NP_080669
• UniProt ID: Q8K2T1