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Recombinant Mouse Nono Protein, Myc/DDK-tagged

Cat.No. : Nono-4458M
Product Overview : Purified recombinant protein of mouse full-length non-POU-domain-containing, octamer binding protein (Nono), with C-terminal MYC/DDK tag, expressed in HEK293T cells.
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Description : DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Important for the functional organization of GABAergic synapses. Plays a specific and important role in the regulation of synaptic RNAs and GPHN/gephyrin scaffold structure, through the regulation of GABRA2 transcript.Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.
Source : HEK293T
Species : Mouse
Tag : Myc/DDK
Molecular Mass : 54.5 kDa
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade after receiving vials.
Concentration : >50 μg/mL as determined by microplate BCA method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Gene Name : Nono non-POU-domain-containing, octamer binding protein [ Mus musculus (house mouse) ]
Official Symbol : Nono
Synonyms : Nono; non-POU-domain-containing, octamer binding protein; P54; nonA; NRB54; P54NRB; AA407051; AV149256; non-POU domain-containing octamer-binding protein
Gene ID : 53610
mRNA Refseq : NM_023144
Protein Refseq : NP_075633
UniProt ID : Q99K48

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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In which tissues or organs is NONO protein highly expressed? 06/15/2022

This protein is widely expressed in a variety of tissues and organs, including the brain, heart, liver, kidneys, and muscles.

Is NONO protein involved in RNA splicing regulation? 03/24/2022

Yes, NONO protein is involved in the regulation of RNA splicing and plays an important role in the splicing process by interacting with other proteins.

Is NONO protein involved in liver function? 02/08/2022

NONO protein is expressed in the liver and is involved in the regulation of signaling pathways related to liver function. However, further research is needed to determine its specific role.

Is there a relationship between NONO protein and DNA damage repair? 01/10/2022

NONO protein interacts with DNA repair-related proteins and is involved in the repair process of DNA double-strand breaks.

What is the role of NONO protein in neurological diseases? 11/30/2021

The protein has been implicated in some neurological diseases, such as motor neuron disease, but its specific role and mechanism still need to be further studied.

What diseases are NONO associated with? 01/21/2021

NONO protein has been implicated in a variety of diseases, including leukemia, neurological diseases such as motor neuron disease, and progeria.

Customer Reviews (3)

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Reviews
08/08/2023

    The stability of NONO has good adaptability under different temperature and pH conditions.

    04/01/2022

      NONO has important application value in the field of drug research and development, and has achieved certain research results.

      04/01/2020

        Experiments with NONO are easy to use and do not require complex procedures.

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