Active Recombinant Mouse P4hb protein, His-tagged

• Cat.No.: P4hb-1130M
• Product Overview: Recombinant Mouse P4hb protein(NP_035162.1)(Met1-Lys506), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is > 7.5 A650/min/mg
• Molecular Mass: The secreted recombinant mouse P4HB comprises 498 amino acids and has a calculated molecular mass of 56.2 kDa as estimated in SDS-PAGE under reducing conditions.
• Protein Length: Met1-Lys506
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: P4hb prolyl 4-hydroxylase, beta polypeptide [ Mus musculus ]
• Official Symbol: P4hb
• Synonyms: P4HB; prolyl 4-hydroxylase, beta polypeptide; protein disulfide-isomerase; p55; ER protein 59; protein disulfide isomerase; prolyl 4-hydroxylase subunit beta; cellular thyroid hormone-binding protein; endoplasmic reticulum resident protein 59; PDI; Thbp; ERp59; Pdia1
• Gene ID: 18453
• mRNA Refseq: NM_011032
• Protein Refseq: NP_035162