Recombinant Mouse Rgma Protein, Myc/DDK-tagged

• Cat.No.: Rgma-5488M
• Product Overview: Purified recombinant protein of mouse full-length RGM domain family, member A (cDNA clone MGC:38550 IMAGE:5353958), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 47.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rgma repulsive guidance molecule family member A [ Mus musculus (house mouse) ]
• Official Symbol: Rgma
• Synonyms: Rgma; repulsive guidance molecule family member A; BC059072; C230063O06; repulsive guidance molecule A; RGM domain family, member A
• Gene ID: 244058
• mRNA Refseq: NM_177740
• Protein Refseq: NP_808408
• UniProt ID: Q6PCX7