"SCF" Related Products

Recombinant Mouse SCF Protein

Cat.No.: SCF-65M
Product Overview: Recombinant mouse SCF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Description: Stem Cell Factor (SCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. SCF binds to and activates the SCF receptor (SCFR), a receptor tyrosine kinase. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell-cell adhesion and acts synergistically with other cytokine.
Source: E. coli
Species: Mouse
Form: Liquid. In Phosphate buffer saline (pH 7.4) containing 10% glycerol.
Bio-activity: Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 for this effect is less or equal to 20 ng/ml.
Molecular Mass: 18 kDa (165 aa), confirmed by MALDI-TOF
AA Sequence: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
Purity: >95% by SDS - PAGE
Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
Concentration: 1 mg/ml (determined by Bradford assay)
Gene Name: Kitl kit ligand [ Mus musculus ]
Official Symbol: Kitl
Synonyms: Kitl; Clo; Con; SF; Sl; SLF; Steel; SCF
Gene ID: 17311
mRNA Refseq: NM_013598
Protein Refseq: NP_038626
MIM:
UniProt ID: P20826
Chromosome Location: 10 D1; 10 51.4 cM
Function: cytokine activity; growth factor activity; stem cell factor receptor binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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