Recombinant Mouse Ska3 Protein, Myc/DDK-tagged

• Cat.No.: Ska3-5892M
• Product Overview: Purified recombinant protein of mouse full-length spindle and kinetochore associated complex subunit 3 (Ska3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 45.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ska3 spindle and kinetochore associated complex subunit 3 [ Mus musculus (house mouse) ]
• Official Symbol: Ska3
• Synonyms: Ska3; spindle and kinetochore associated complex subunit 3; F630043A04Rik; spindle and kinetochore-associated protein 3
• Gene ID: 219114
• mRNA Refseq: NM_198605
• Protein Refseq: NP_941007
• UniProt ID: Q8C263