Recombinant Mouse Sptlc1 Protein, Myc/DDK-tagged

• Cat.No.: Sptlc1-02M
• Product Overview: Purified recombinant protein of Mouse serine palmitoyltransferase, long chain base subunit 1 (Sptlc1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates. The SPTLC1-SPTLC2-SPTSSB complex displays a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme has the ability to use a broader range of acyl-CoAs. Required for adipocyte cell viability and metabolic homeostasis.
• Source: HEK293T
• Species: Mouse
• Tag: C-Myc/DDK
• Molecular Mass: 53 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Sptlc1 serine palmitoyltransferase, long chain base subunit 1 [ Mus musculus (house mouse) ]
• Official Symbol: Sptlc1
• Synonyms: Sptlc1; serine palmitoyltransferase, long chain base subunit 1; Sp; Lcb; Lcb1; SPT1; C77762; AW552086; E030036H05; serine palmitoyltransferase 1; long chain base biosynthesis protein 1; serine-palmitoyl-CoA transferase 1; EC 2.3.1.50
• Gene ID: 268656
• mRNA Refseq: NM_009269
• Protein Refseq: NP_033295
• UniProt ID: O35704