Recombinant Mouse TF Protein, MIgG2a Fc-tagged, low endotoxin

Cat.No.: TF-379M
Product Overview: Recombinant Mouse TF Protein, MIgG2a Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Val 20 - His 697 (Accession # Q921I1-1). Predicted N-terminus: Val 20. This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 101.8 kDa. The protein migrates as 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Source: HEK293
Species: Mouse
Tag : MIgG2a Fc
Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arg, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Bio-activity: Immobilized Human TfR, His Tag at 2 μg/mL (100 μL/well) can bind Recombinant Mouse TF Protein, MIgG2a Fc-tagged, low endotoxin with a linear range of 0.4-12.5 ng/mL (QC tested).
Molecular Mass: 101.8 kDa
Protein length: Val 20 - His 697
Endotoxin: Less than 0.1 EU per μg by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Notes: Please avoid repeated freeze-thaw cycles.
Stability: No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

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