Active Recombinant Mouse Tlr5 protein, Fc-tagged

• Cat.No.: Tlr5-392M
• Product Overview: Recombinant Mouse Tlr5(Ile21-Phe641) fused with Fc tag at C-terminal was expressed in CHO cells.

Specification

• Source: CHO
• Species: Mouse
• Tag: Fc
• Predicted N Terminal: Ile21
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit Flagellin-induced IL-8 secretion in HT-29 human colon adenocarcinoma cells. The ED50 for this effect is typically 4-24 ng/Ml
• Molecular Mass: Predicted Molecular Mass: 97.3 (monomer) kDa;SDS-PAGE: 100-125 kDa, reducing conditions.
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.

Gene Information

• Gene Name: Tlr5 toll-like receptor 5 [ Mus musculus ]
• Official Symbol: Tlr5
• Synonyms: TLR5; toll-like receptor 5
• Gene ID: 53791
• mRNA Refseq: NM_016928
• Protein Refseq: NP_058624
• UniProt ID: Q9JLF7
• Chromosome Location: 1 H5; 1 86.12 Cm
• Pathway: Legionellosis, organism-specific biosystem; Legionellosis, conserved biosystem; Salmonella infection, organism-specific biosystem; Salmonella infection, conserved biosystem; Toll-like receptor signaling pathway, organism-specific biosystem; Toll-like receptor signaling pathway, conserved biosystem; mRNA processing, organism-specific biosystem
• Function: interleukin-1 receptor binding; receptor activity