Recombinant Mouse Trim33 Protein, Myc/DDK-tagged

• Cat.No.: Trim33-6654M
• Product Overview: Purified recombinant protein of mouse full-length tripartite motif-containing 33 (Trim33), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor. Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 124.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Trim33 tripartite motif-containing 33 [ Mus musculus (house mouse) ]
• Official Symbol: Trim33
• Synonyms: TRIM33; tripartite motif-containing 33; E3 ubiquitin-protein ligase TRIM33; TIF1-gamma; ectodermin homolog; tripartite motif protein 33; tripartite motif-containing protein 33; transcription intermediary factor 1-gamma; Ecto; Tif1g; AI413936; mKIAA1113; ectodermin; 8030451N04Rik; DKFZp586K1123
• Gene ID: 94093
• mRNA Refseq: NM_053170
• Protein Refseq: NP_444400
• UniProt ID: Q99PP7