Recombinant Mouse Tspan12 Protein, Myc/DDK-tagged

• Cat.No.: Tspan12-6689M
• Product Overview: Purified recombinant protein of mouse full-length tetraspanin 12 (Tspan12), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Regulator of cell surface receptor signal transduction. Acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. Activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP). Activates MMP14/MT1-MMP-dependent cleavage activity. Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. Suprisingly, it only activate the norrin (NDP)-dependent activation of FZD4, while it does not activate the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1).
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 35.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Tspan12 tetraspanin 12 [ Mus musculus (house mouse) ]
• Official Symbol: Tspan12
• Synonyms: TSPAN12; tetraspanin 12; tetraspanin-12; tspan-12; transmembrane 4 superfamily member 12; Tm4sf12; AI426782; AI663988; AW111457; 9030619E17
• Gene ID: 269831
• mRNA Refseq: NM_173007
• Protein Refseq: NP_766595
• UniProt ID: Q8BKT6