Recombinant Mouse Uba5 Protein, Myc/DDK-tagged

• Cat.No.: Uba5-6767M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin-like modifier activating enzyme 5 (Uba5), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: E1-like enzyme which specifically catalyzes the first step in ufmylation. Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP. Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer. Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding. Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism. Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress. Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 44.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Uba5 ubiquitin-like modifier activating enzyme 5 [ Mus musculus (house mouse) ]
• Official Symbol: Uba5
• Synonyms: UBA5; ubiquitin-like modifier activating enzyme 5; ubiquitin-like modifier-activating enzyme 5; UFM1-activating enzyme; ubiquitin-activating enzyme 5; ubiquitin-activating enzyme E1-domain containing 1; ubiquitin-activating enzyme E1 domain-containing protein 1; Ube1dc1; AW240750; 5730525G14Rik
• Gene ID: 66663
• mRNA Refseq: NM_025692
• Protein Refseq: NP_079968
• UniProt ID: Q8VE47