|Product Overview:||Recombinant Mouse VEGF164 Protein is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 190 (Accession # AAB22253.1 ). Predicted N-terminus: Ala 27. This protein carries no "tag". The protein has a calculated MW of 19.3 kDa. The protein migrates as 23-25 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.|
|Form:||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.|
|Bio-activity:||Immobilized Recombinant Mouse VEGF164 Protein at 2 μg/mL (100 μL/well) can bind VEGFR1/R2-Fc with a linear range of 0.6-2.5 ng/mL (QC tested).|
|Molecular Mass:||19.3 kDa|
|Protein length:||Ala 27 - Arg 190|
|Endotoxin:||Less than 1.0 EU per μg by the LAL method.|
|Purity:||>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
|Notes:||Please avoid repeated freeze-thaw cycles.|
|Stability:||No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage:||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.|
|Reconstitution:||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|
|Gene Name:||Vegfa vascular endothelial growth factor A [ Mus musculus ]|
|Synonyms:||VEGFA; vascular endothelial growth factor A; vascular permeability factor; Vpf; Vegf; Vegf120; Vegf164; Vegf188;|