Active Recombinant Paguma larvata ACE2 protein, hFc-tagged

• Cat.No.: ACE2-019P
• Product Overview: Recombinant Paguma larvata ACE2 protein(Q56NL1)(18-740aa), fused with C-terminal hFc tag, was expressed in Mammalian cell.

Specification

• Source: Mammalian cell
• Species: Paguma larvata
• Tag: hFc
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 2 μg/ml can bind Paguma larvata ACE2, the EC50 is 5.056-7.559 ng/ml. Recombinant Paguma larvata ACE2 protein enzyme activity is measured by its ability to cleave fluorogenic peptide substrate(Mca-Ala-Pro-Lys(Dnp)-OH), The Km is 22.84 μM.
• Molecular Mass: 112.7 kDa
• Protein length: 18-740aa
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Purity: Greater than 94.8% as determined by SDS-PAGE.
• Storage: Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.