Active Recombinant Pig CYP2E1 Protein
Cat.No. : | CYP2E1-134P |
Product Overview : | Recombinant pig CYP2E1 protein and CYP-reductase without tag were coexpressed in E. coli. |
- Specification
- Gene Information
- Related Products
Description : | A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics. |
Source : | E. coli |
Species : | Pig |
Bio-activity : | Cytochrome c Reductase Activity: 367 nmol/min/mg protein P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase]) Activity: Chlorzoxazone 6-hydroxylase Linear with time up to: 15 min(Determined at 100 µM substrate, using a P450 concentration of 100 pmol/ml) Linear with P450 up to: 160 pmol/ml(Determined at 100 µM substrate, using an incubation time of 5 min) Vmax: 3.2 pmol/min/pmol CYP Km: 132 µM |
Usage : | For laboratory (research) purposes only |
Storage : | Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice. |
Concentration : | P450 Concentration: 9.9 nmol/ml Cytochrome b5 Concentration: 49.4 nmol/ml Protein Concentration: 17.7 mg/ml |
Storage Buffer : | 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA |
Gene Name : | CYP2E1 cytochrome P450, family 2, subfamily E, polypeptide 1 [ Sus scrofa (pig) ] |
Official Symbol : | CYP2E1 |
Synonyms : | CYP2E1; cytochrome P450, family 2, subfamily E, polypeptide 1; cytochrome P450 2E1; 4-nitrophenol 2-hydroxylase; CYPIIE1; cytochrome P-450-J; cytochrome P450-J |
Gene ID : | 403216 |
mRNA Refseq : | NM_214421 |
Protein Refseq : | NP_999586 |
UniProt ID : | P79383 |
Products Types
◆ Recombinant Protein | ||
CYP2E1-221H | Active Recombinant Human CYP2E1 Protein | +Inquiry |
CYP2E1-430H | Recombinant Human CYP2E1 Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
CYP2E1-0005H | Recombinant Human CYP2E1 Protein (L32-S493), Tag Free | +Inquiry |
Cyp2e1-2417M | Recombinant Mouse Cyp2e1 Protein, Myc/DDK-tagged | +Inquiry |
CYP2E1-220H | Active Recombinant Human CYP2E1 Protein | +Inquiry |
◆ Lysates | ||
CYP2E1-7111HCL | Recombinant Human CYP2E1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (7)
Ask a questionGenetic polymorphisms in CYP2E1 can result in altered enzyme activity, influencing an individual's response to drugs and susceptibility to liver damage.
CYP2E1 oxidizes and metabolizes various small molecules, including drugs and toxins.
It plays a significant role in ethanol metabolism and helps detoxify industrial solvents and other xenobiotics.
Ethanol, fasting, and certain drugs can induce the expression of CYP2E1.
CYP2E1 activity generates ROS, contributing to oxidative stress and potential cellular damage.
CYP2E1 is highly expressed in the liver, which is the main site for drug metabolism.
CYP2E1 can contribute to drug-drug interactions, affecting the efficacy and toxicity of co-administered drugs.
Customer Reviews (3)
Write a reviewProduct is as described.
Fast and efficient service.
Great for biological experiments.
Ask a Question for All CYP2E1 Products
Required fields are marked with *
My Review for All CYP2E1 Products
Required fields are marked with *
Inquiry Basket