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Active Recombinant Pig CYP2E1 Protein

Cat.No. : CYP2E1-134P
Product Overview : Recombinant pig CYP2E1 protein and CYP-reductase without tag were coexpressed in E. coli.
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  • Gene Information
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Description : A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.
Source : E. coli
Species : Pig
Bio-activity : Cytochrome c Reductase Activity: 367 nmol/min/mg protein
P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
Activity: Chlorzoxazone 6-hydroxylase
Linear with time up to: 15 min(Determined at 100 µM substrate, using a P450 concentration of 100 pmol/ml)
Linear with P450 up to: 160 pmol/ml(Determined at 100 µM substrate, using an incubation time of 5 min)
Vmax: 3.2 pmol/min/pmol CYP
Km: 132 µM
Usage : For laboratory (research) purposes only
Storage : Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration : P450 Concentration: 9.9 nmol/ml
Cytochrome b5 Concentration: 49.4 nmol/ml
Protein Concentration: 17.7 mg/ml
Storage Buffer : 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Gene Name : CYP2E1 cytochrome P450, family 2, subfamily E, polypeptide 1 [ Sus scrofa (pig) ]
Official Symbol : CYP2E1
Synonyms : CYP2E1; cytochrome P450, family 2, subfamily E, polypeptide 1; cytochrome P450 2E1; 4-nitrophenol 2-hydroxylase; CYPIIE1; cytochrome P-450-J; cytochrome P450-J
Gene ID : 403216
mRNA Refseq : NM_214421
Protein Refseq : NP_999586
UniProt ID : P79383

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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Are there known genetic polymorphisms in CYP2E1 associated with altered drug metabolism or disease risk? 05/03/2022

Genetic polymorphisms in CYP2E1 can result in altered enzyme activity, influencing an individual's response to drugs and susceptibility to liver damage.

What is the main function of CYP2E1 in drug metabolism? 10/19/2021

CYP2E1 oxidizes and metabolizes various small molecules, including drugs and toxins.

How does CYP2E1 contribute to the metabolism of ethanol and other toxins? 02/27/2021

It plays a significant role in ethanol metabolism and helps detoxify industrial solvents and other xenobiotics.

What factors can induce the expression of CYP2E1? 08/08/2020

Ethanol, fasting, and certain drugs can induce the expression of CYP2E1.

How is CYP2E1 linked to the generation of reactive oxygen species (ROS)? 11/15/2019

CYP2E1 activity generates ROS, contributing to oxidative stress and potential cellular damage.

In which tissues is CYP2E1 predominantly expressed? 01/24/2019

CYP2E1 is highly expressed in the liver, which is the main site for drug metabolism.

What role does CYP2E1 play in drug-drug interactions? 08/05/2018

CYP2E1 can contribute to drug-drug interactions, affecting the efficacy and toxicity of co-administered drugs.

Customer Reviews (3)

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Reviews
07/04/2022

    Product is as described.

    04/12/2022

      Fast and efficient service.

      12/19/2020

        Great for biological experiments.

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