Active Recombinant Porcine IL2 Protein

• Cat.No.: IL2-83P
• Product Overview: Recombinant Porcine IL2 was expressed in E. coli.

Specification

• Description: IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
• Source: E. coli
• Species: Porcine
• Form: The protein (1mg/ml) was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4.
• Bio-activity: The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.5ng/ml, corresponding to a specific activity of > 2.0 × 10^6 IU/mg.
• Molecular Mass: 15217 Dalton
• Protein length: 134 amino acids
• AA Sequence: APTSSSTKNT KKQLEPLLLD LQLLLKEVKN YENADLSRML TFKFYMPKQA TELKHLQCLV EELKALEGVL NLGQSKNSDS ANIKESMNNI NVTVLELKGS ETSFKCEYDD ETVTAVEFLN KWITFCQSIY STLT.
• Purity: Greater than 96.0% as determined by:
  (a) Analysis by RP-HPLC.
  (b) Analysis by SDS-PAGE.
• Storage: Lyophilized IL2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution IL-2 should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade.
  For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  Please prevent freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized IL 2 in sterile distilled H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Gene Information

• Gene Name: IL2 interleukin 2 [ Sus scrofa ]
• Official Symbol: IL2
• Synonyms: IL-2; TCGF; POIL2; T-cell growth factor
• Gene ID: 396868
• mRNA Refseq: NM_213861
• Protein Refseq: NP_999026
• UniProt ID: P26891