Active Recombinant Rat Cyp2c11 Protein

• Cat.No.: Cyp2c11-142R
• Product Overview: Recombinant rat Cyp2c11 protein and CYP-reductase without tag were coexpressed in E. coli.

Specification

• Description: Most abundant member of the p450 xenobiotic-inducible superfamily in non-stimulated liver; metabolizes xenobiotics and testosterone
• Source: E. coli
• Species: Rat
• Bio-activity: Cytochrome c Reductase Activity: 2347 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Testosterone 6β-hydroxylase
  Linear with time up to: 12 min(Determined at 5 μM substrate, using a P450 concentration of 6.25 pmol/ml)
  Linear with P450 up to: 25 pmol/ml(Determined at 5 μM substrate, using an incubation time of 5 min)
  Vmax: 17 pmol/min/pmol CYP
  Km: 4.4 µM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 1.9 nmol/ml
  Protein Concentration: 9.5 mg/ml
  Specific P450 Content: 200 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: Cyp2c11 cytochrome P450, subfamily 2, polypeptide 11 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Cyp2c11
• Synonyms: Cyp2c11; cytochrome P450, subfamily 2, polypeptide 11; Cyp2c; CYP2CII; Cyp2c11l; cytochrome P450 2C11; CYPIIC11; P-450(M-1); P450-UT-A; P450H; UT-2; cytochrome P-450(M-1); cytochrome P450 2C11-like; cytochrome P450, 2c29; cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase), polypeptide 11; cytochrome P450-UT-2; cytochrome P450-UT-A; cytochrome P450H
• Gene ID: 29277
• mRNA Refseq: NM_019184
• Protein Refseq: NP_062057
• UniProt ID: P08683