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Active Recombinant Rat Defb4

Cat.No. : Defb4-8655R
Product Overview : Recombinant Rat Defb4 was expressed in E. coli.
  • Specification
  • Gene Information
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Description : Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, four rat β-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. β-defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. BD-4 is expressed in testis, stomach, uterus, neutrophils, thyroid, lung and kidney.
Source : E. coli
Species : Rat
Form : Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, pH 7.4, 500mM NaCl.
Bio-activity : Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 0.1-100.0 ng/ml.
Molecular Mass : Approximately 4.4 KDa, a single non-glycosylated polypeptide chain containing 41 amino acids.
AA Sequence : QSINNPITCL TKGGVCWGPC TGGFRQIGTC GLPRVRCCKK K
Endotoxin : Less than 1EU/μg of rRtBD-4 as determined by LAL method.
Purity : >95% by SDS-PAGE and HPLC analyses.
Storage : This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at
Gene Name : Defb4 defensin beta 4 [ Rattus norvegicus ]
Official Symbol : Defb4
Synonyms : DEFB4; defensin beta 4; beta-defensin 4; BD-2; BD-4; RBD-2; RBD-4; beta defensin-2; defensin beta 3; Defb2; Defb3;
Gene ID : 64389
mRNA Refseq : NM_022544
Protein Refseq : NP_071989

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Is the expression of DEFB4 affected by environmental factors? 11/16/2022

Yes, the expression of DEFB4 is affected by environmental factors, such as nutritional status, inflammation level, microbial infection, etc.

How to regulate DEFB4 expression? 07/17/2022

DEFB4 expression can be regulated by techniques such as gene knockout, gene silencing, or gene transduction. In addition, environmental factors (e.g., nutrition, inflammation, etc.) can also affect its expression levels.

Are there other coding variants of DEFB4? 03/10/2021

Yes, there are other coding variants of DEFB4 that may have different functions and expression patterns.

What is the significance of studying DEFB4? 08/11/2020

Studying DEFB4 can help to gain insight into the mechanisms of immune response and inflammatory response, which is of great significance for the development of new drugs and therapeutic strategies. In addition, it is also of great value for understanding the pathogenesis and prevention of diseases.

What is the relationship between DEFB4 and muscle atrophy? 04/16/2020

Studies have shown that DEFB4 can inhibit the occurrence of muscle atrophy, but the specific mechanism is not yet clear.

What are the applications of DEFB4 in drug discovery? 12/01/2019

DEFB4 can be used as a target for the development of new drugs, or as a drug carrier for the directional delivery of drugs.

Customer Reviews (3)

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Reviews
04/15/2022

    Under the influence of temperature, humidity and light, this protein is stable and durable.

    03/29/2022

      DEFB4 is immunogenic and allergenic, which is even more important for proteins used in drug development.

      05/02/2021

        The consistency and reliability of this protein are good.

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