Active Recombinant Rat EPO, His-tagged
Cat.No. : | EPO-578R |
Product Overview : | Recombinant Rat EPO (P29676-1) (Met 1-Arg 192), fused with a polyhistidine tag at the C-terminus, was produced in Baculovirus-Insect cells. |
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Source : | Insect cells |
Species : | Rat |
Tag : | His |
Form : | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Measured by its ability to bind human EPOR-Fc in a functional ELISA. 2. Measured by its ability to bind biotinylated human EPOR-His in a functional ELISA. 3. Measured by its ability to bind mouse EPOR-Fch in a functional ELISA. 4. Measured by its ability to bind human EPOR & CD131-Fc in a functional ELISA. 5. Measured by its ability to inhibit Epo-dependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is 2-10ng/mL. |
Molecular Mass : | The recombinant rat EPO comprises 176 amino acids with and has a predicted molecular mass of 20 kDa. It migrates as an approximately 27 kDa band in reduced SDS-PAGE. |
Protein length : | Met 1-Arg 192 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 90 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | Epo erythropoietin [ Rattus norvegicus ] |
Official Symbol : | EPO |
Gene ID : | 24335 |
mRNA Refseq : | NM_017001.1 |
Protein Refseq : | NP_058697.1 |
Products Types
◆ Recombinant Protein | ||
Epo-1848R | Recombinant Rat Epo Protein | +Inquiry |
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Epo-964M | Active Recombinant Mouse Epo Protein | +Inquiry |
EPO-124E | Active Recombinant Human EPO alpha Protein (165 aa) | +Inquiry |
◆ Lysates | ||
EPO-1450MCL | Recombinant Mouse EPO cell lysate | +Inquiry |
EPO-592RCL | Recombinant Rat EPO cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-0327 | Eosinophil Peroxidase Fluorometric Detection Kit | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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