Active Recombinant Rat Interleukin-17E (IL-25)

• Cat.No.: IL25-495R
• Product Overview: Recombinant rat IL-17E produced in E.coli is a nonglycosylated disulfide-joined homodimer having a molecular mass of 35.5 kDa.

Specification

• Cat. No.: IL25-495R
• Description: Rat IL-17E (IL-17), is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Rat IL-17E protein is a homodimer consisting of two 145 amino acids peptides.
• Species: Rat
• Source: E.coli
• Form: Lyophilized with no additives.
• Purity: Purity Greater than 97% as determined by: Reducing and non-reducing SDS-PAGE.
• Bio-activity: Rat IL-17E activity is measured by the dose-dependent induction of mG-CSF production in cultured NIH 3T3 cells. The dose range for this activity is 10-100ng/ml.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized product with sterile 20 mM HCl at a concentration of 0.1 mgμs/ml. Further dilutions into other buffers can be made.
• Purity Greater than 97% as determined by: Reducing and non-reducing SDS-PAGE.
• Protein Content determined by: UV spectroscopy at 280 nm. Quantitation on SDS-PAGE against a known standard.
• Endotoxin Level: Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
• Amino acid sequence: CSHLPRCCPS KQQEFPEEWL KWNPAPVSPP EPLRHTHHPE SCRASKDGPL NSRAISPWSY ELDRDLNRVP QDLYHARCLC PHCVSLQTGS HMDPMGNSVP LYHNQTVFYR RPCHGEQGAH GRYCLERRLY RVSLACVCVR PRMMA
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Gene Information

• Gene Name: Il25 interleukin 25 [ Rattus norvegicus ]
• Official Symbol: IL25
• Synonyms: Il25; interleukin 25; RGD1561632; rgd1561632_predicted; similar to IL25; similar to il25 (predicted); similar to IL25 (predicted); RGD1561632_predicted; LOC501996
• Gene ID: 501996
• mRNA Refseq: XM_577423
• Protein Refseq: XP_577423
• Chromosome Location: 15p13
• Pathway: Cytokine-cytokine receptor interaction
• Function: cytokine activity