| Cat. No. : |
IL4-411R |
| Description : |
IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| Source : |
Rat |
| Host : |
Escherichia Coli. |
| Form : |
Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. |
| Purity : |
Greater than 98.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Bio-activity : |
The ED50 as determined by the dose-dependant prolifiration of Con A activated rat spleen cells is < 1.5 ng/ml, corresponding to a Specific Activity of 660,000 IU/mg. |
| Physical Appearance : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Solubility : |
It is recommended to reconstitute the lyophilized Interleukin 4 in sterile 18Mμ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Amino acid sequence : |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Gly-Cys-Asn. |
| Storage : |
Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
