Recombinant Rat Unc5b Protein, hIgG/His-tagged

• Cat.No.: Unc5b-039R
• Product Overview: Recombinant Rat UNC5B/UNC5B, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specification

• Description: Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. Functions as netrin receptor that negatively regulates vascular branching during angiogenesis. Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity.
• Source: HEK293
• Species: Rat
• Tag: hIgG/His
• Form: Liquid
• Molecular Mass: 66.0 kDa
• AA Sequence: GIDSGGQALPDSFPSAPAEQLPHFL LEPEDAYIVKNKPVELHCRAFPATQ IYFKCNGEWVSQKGHVTQESLDEAT GLRIREVQIEVSRQQVEELFGLEDY WCQCVAWSSSGTTKSRRAYIRIAYL RKNFDQEPLAKEVPLDHEVLLQCRP PEGVPVAEVEWLKNEDVIDPAQDTN FLLTIDHNLIIRQARLSDTANYTCV AKNIVAKRRSTTATVIVYVNGGWSS WAEWSPCSNRCGRGWQKRTRTCTNP APLNGGAFCEGQACQKTACTTVCPV DGAWTEWSKWSACSTECAHWRSREC MAPPPQNGGRDCSGTLLDSKNCTDG LCVLNQRTLNDPKSRPLEPSGD
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -118 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: Unc5b unc-5 netrin receptor B [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Unc5b
• Synonyms: Unc5b; unc-5 netrin receptor B; Unc5h2; netrin receptor UNC5B; protein unc-5 homolog 2; protein unc-5 homolog B; transmembrane receptor Unc5H2; unc-5 homolog 2; unc-5 homolog B
• Gene ID: 60630
• mRNA Refseq: NM_022207
• Protein Refseq: NP_071543
• UniProt ID: O08722