|Product Overview:||Recombinant Rhesus Monkey FLT1 Protein, MIgG2a Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Asn 756 (Accession # F7FAY2-1). Predicted N-terminus: Ser 27. This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 109.1 kDa. The protein migrates as 130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Tag :||MIgG2a Fc|
|Form:||Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.|
|Bio-activity:||Immobilized Rhesus macaque PLGF at 0.5 μg/mL (100 μL/well) can bind Recombinant Rhesus Monkey FLT1 Protein, MIgG2a Fc-tagged, low endotoxin with a linear range of 0.6-10 ng/mL (QC tested).
Immobilized Recombinant Rhesus Monkey FLT1 Protein, MIgG2a Fc-tagged, low endotoxin at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165, His Tag with a linear range of 0.5-3.9 ng/mL (Routinely tested).
|Molecular Mass:||109.1 kDa|
|Protein length:||Ser 27 - Asn 756|
|Endotoxin:||Less than 0.1 EU per μg by the LAL method.|
|Purity:||>95% as determined by SDS-PAGE.|
|Notes:||Please avoid repeated freeze-thaw cycles.|
|Stability:||No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage:||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.|
|Reconstitution:||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|