| Description : |
Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation. |
| Source : |
E.coli |
| Species : |
Streptolysin |
| Form : |
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. |
| Molecular Mass : |
Approximately 60.1 kDa, a single non-glycosylated polypeptide chain containing 538 amino acids. |
| Protein length : |
538 |
| AA Sequence : |
NKQNTASTETTTTNEQPKPESSELT TEKAGQKTDDMLNSNDMIKLAPKEM PLESAEKEEKKSEDKKKSEEDHTEE INDKIYSLNYNELEVLAKNGETIEN FVPKEGVKKADKFIVIERKKKNINT TPVDISIIDSVTDRTYPAALQLANK GFTENKPDAVVTKRNPQKIHIDLPG MGDKATVEVNDPTYANVSTAIDNLV NQWHDNYSGGNTLPARTQYTESMVY SKSQIEAALNVNSKILDGTLGIDFK SISKGEKKVMIAAYKQIFYTVSANL PNNPADVFDKSVTFKELQRKGVSNE APPLFVSNVAYGRTVFVKLETSSKS NDVEAAFSAALKGTDVKTNGKYSDI LENSSFTAVVLGGDAAEHNKVVTKD FDVIRNVIKDNATFSRKNPAYPISY TSVFLKNNKIAGVNNRTEYVETTST EYTSGKINLSHQGAYVAQYEILWDE INYDDKGKEVITKRRWDNNWYSKTS PFSTVIPLGANSRNIRIMARECTGL AWEWWRKVIDERDVKLSKEINVNIS GSTLSPYGSITYK |
| Endotoxin : |
Less than 0.1 EU/μg of rStreptolysin O as determined by LAL method. |
| Purity : |
>97% by SDS-PAGE and HPLC analysis. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
