Recombinant Human POLE protein

• Cat.No.: POLE-1522H
• Product Overview: Recombinant human DNA polymerase epsilon catalytic fragment (wild type, exo-proficient) expressed in E. coli.

Specification

• Description: DNA polymerase epsilon is a member of B family DNA polymerases. It is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have recently been found associated with colorectal cancer.
• Source: E. coli
• Species: Human
• Form: 50 mM Tris-HCl (pH7.5), 500 mM NaCl, and 10% glycerol.
• Molecular Mass: 168 kDa
• Usage: Suggested reaction buffer (not included): 50 mM Tris-HCl (pH7.4), 8 mM MgCl2, 1 mM DTT, 10% glycerol, 200 M dNTPs, 100 nM DNA primer-template, and, 2 nM -10nM pol epsilon.
  Suggested dilution buffer (not included): 50 mM Tris-HCl (pH 7.5), 5 mM-mercaptoethanol, 50% glycerol.
  Reaction temperature: 37 centigrade
  Note: Reaction can be stopped by adding equal volume of 95% formamide.
• Storage: Stable for 2 years at -70 centigrade from date of
  shipment. Please aliquot to avoid repeated
  freezing and thawing.

Gene Information

• Gene Name: POLE polymerase (DNA directed), epsilon [ Homo sapiens ]
• Official Symbol: POLE
• Synonyms: POLE; polymerase (DNA directed), epsilon; DNA polymerase epsilon catalytic subunit A; POLE1; DNA polymerase II subunit A; FLJ21434; DKFZp434F222
• Gene ID: 5426
• mRNA Refseq: NM_006231
• Protein Refseq: NP_006222
• MIM: 174762
• UniProt ID: Q07864