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SYK Activity Assay Kit

Cat.No. : Kit-1625
Product Overview : CTK Activity cell lines are engineered to the catalytic domain from CTK of interest fused to the cytokine receptor binding domain (CRBD) of a JAK kinase. The cells co-an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the receptor-PK. Activation of the cytokine receptor-PK induces receptor phosphorylation by the CTK catalytic domain. This leads to SH2-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.
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Size : 100 dp(1 x 96-well)/200 dp(2 x 96-well)/1000 dp(10 x 96-well)
Storage : Short term (2 weeks): Store in vapor phase of liquid N2

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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