ANAPC5
-
Official Full Name
anaphase promoting complex subunit 5
-
Overview
APC5 (anaphase-promoting complex subunit 5) is a member of the E3 enzyme family. This protein has a molecular weight of approximately 79 kD. The APC5 protein is located in the nucleus during interphase and at the centrosome during metaphase/anaphase. This -
Synonyms
ANAPC5; anaphase promoting complex subunit 5; anaphase-promoting complex subunit 5; APC5; cyclosome subunit 5;
- Recombinant Proteins
- Cell & Tissue Lysates
- Protein Pre-coupled Magnetic Beads
- Chicken
- Human
- Mouse
- Rat
- Zebrafish
- E.coli
- E.Coli or Yeast
- HEK293
- HEK293T
- In Vitro Cell Free System
- Mammalian Cell
- Mammalian cells
- Wheat Germ
- Flag
- GST
- His
- His (Fc)
- Avi
- Myc
- DDK
- Myc|DDK
- N/A
- Involved Pathway
- Protein Function
- Interacting Protein
ANAPC5 involved in several pathways and played different roles in them. We selected most pathways ANAPC5 participated on our site, such as Cell cycle, Oocyte meiosis, Ubiquitin mediated proteolysis, which may be useful for your reference. Also, other proteins which involved in the same pathway with ANAPC5 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
Pathway Name | Pathway Related Protein |
---|---|
Cell cycle | NDC80;CHEK2;DHFR;GADD45AB;TUBGCP2;MAPRE1A;MCM8;SET;CDK11B |
Oocyte meiosis | CPEB1B;YWHAE2;MAPK12;PRKACA;MAD2L1;CPEB3;CALML3;CALM;REC8B |
Ubiquitin mediated proteolysis | SOCS3B;TRAF6;UBE2S;ANAPC5;BTRC;ANAPC4;UBE2F;TCEB1B;PARK2 |
Progesterone-mediated oocyte maturation | GNAI2;CCNB3;RPS6KA3A;RPS6KA3B;FZR1;PIK3R5;ANAPC7;CCNB1;ANAPC5 |
HTLV-I infection | RB1;TNFRSF13C;POLD4;FOS;PIK3CA;CCND2;FZD6;PRKACG;WNT9A |
ANAPC5 has several biochemical functions, for example, protein phosphatase binding. Some of the functions are cooperated with other proteins, some of the functions could acted by ANAPC5 itself. We selected most functions ANAPC5 had, and list some proteins which have the same functions with ANAPC5. You can find most of the proteins on our site.
Function | Related Protein |
---|---|
protein phosphatase binding | TP53;JUP;PPP1R3G;TRAF3;VRK3;HSF4;TRAF2;ANAPC4;MAPK14 |
ANAPC5 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with ANAPC5 here. Most of them are supplied by our site. Hope this information will be useful for your research of ANAPC5.
CDC27; Cdc23; Cdc16; Cdc26; Anapc16; Cdc20; CDC16
- Q&As
- Reviews
Q&As (23)
Ask a questionWhile the primary function of ANAPC5 is in cell cycle regulation, it is also involved in other cellular processes. ANAPC5 has been implicated in DNA repair, transcriptional regulation, chromatin remodeling, and centrosome duplication.
Yes, ANAPC5 has been implicated in transcriptional regulation. It interacts with transcription factors and co-regulators, suggesting a role in modulating gene expression. ANAPC5 may contribute to the regulation of transcription by influencing the stability and activity of transcription factors and other proteins involved in transcriptional processes.
ANAPC5 is a subunit of the APC/C complex, which plays a key role in the degradation of specific target proteins during cell cycle progression. The APC/C complex recognizes and binds to specific proteins targeted for degradation, marking them for ubiquitination. ANAPC5, along with other subunits, facilitates the transfer of ubiquitin molecules to the target protein, leading to its proteasomal degradation.
Research techniques commonly used to study ANAPC5 include cellular and molecular biology methods, such as immunoprecipitation and co-immunoprecipitation assays, immunofluorescence microscopy, gene expression analysis using techniques like qPCR and RNA sequencing, protein-protein interaction studies, and CRISPR/Cas9-mediated gene editing.
As of now, there are no known small molecules that interact specifically with ANAPC5. However, ongoing research in the field of APC/C complex regulation may identify small molecules that modulate its activity, which could indirectly affect ANAPC5 function.
Yes, ANAPC5 is essential for cell viability. It is a core component of the APC/C complex, which plays a critical role in cell cycle regulation. Depletion or mutation of ANAPC5 can lead to severe defects in cell cycle progression and result in cell death.
Currently, there are no specific inhibitors or modulators that directly target ANAPC5. However, there are inhibitors available that target the APC/C complex as a whole. These inhibitors may indirectly affect ANAPC5 activity within the complex.
While ANAPC5 has been implicated in various diseases, including cancer, it is currently not used as a diagnostic or prognostic marker in clinical practice. Further research is needed to determine its diagnostic or prognostic significance in specific diseases.
Although direct evidence linking ANAPC5 to tumor development is limited, dysregulation of the APC/C complex, in which ANAPC5 plays a crucial role, has been implicated in several types of cancer. Mutations or abnormal expression of other APC/C subunits have been associated with the development of certain malignancies, suggesting a potential role for ANAPC5 in tumorigenesis.
Targeting ANAPC5 directly for therapeutic interventions is challenging due to its essential role in the APC/C complex. However, targeting the APC/C complex as a whole, including ANAPC5, is being explored as a potential therapeutic strategy, particularly in the context of cancer treatment.
ANAPC5 undergoes post-translational modifications such as phosphorylation and ubiquitination. Phosphorylation of ANAPC5 can regulate its stability and activity during specific cell cycle phases, while ubiquitination may play a role in its degradation and turnover.
While no specific diseases or conditions have been directly associated with ANAPC5 mutations, dysregulation of the APC/C complex, including ANAPC5, has been linked to various disorders. These include genetic developmental disorders, chromosome instability syndromes, and certain types of cancer.
Yes, ANAPC5 is highly conserved across different species, indicating its fundamental importance in cellular processes. Its conservation suggests its essential role in the APC/C complex and its involvement in cell cycle regulation and other cellular functions.
Yes, ANAPC5 is involved in cell cycle checkpoints, which are regulatory mechanisms that ensure proper progression through different phases of the cell cycle. The APC/C complex, in which ANAPC5 is a part, controls the activity of key cell cycle regulators and helps enforce checkpoints, such as the G1/S and G2/M checkpoints.
Currently, there are no specific ANAPC5 inhibitors or modulators that have been developed. However, research on targeting the APC/C complex, including ANAPC5, is ongoing as a potential therapeutic strategy for various diseases, including cancer.
ANAPC5 is primarily localized in the nucleus, as it associates with other APC/C subunits to form the APC/C complex. However, during specific stages of the cell cycle, it can translocate to other cellular compartments, such as the centrosomes and spindle poles during mitosis.
Directly targeting ANAPC5 for therapeutic interventions may be challenging due to its essential role in the APC/C complex and its involvement in multiple cellular processes. However, targeting the APC/C complex as a whole has been explored as a potential therapeutic strategy, especially in the context of cancer treatment.
While ANAPC5 primarily functions in cell cycle regulation, it may indirectly influence cell death pathways such as apoptosis. Dysregulation of the cell cycle can lead to aberrant cell proliferation and survival, ultimately impacting cell death decisions. However, further studies are needed to establish a direct link between ANAPC5 and apoptosis.
Currently, there isn't much information regarding specific diseases or conditions directly linked to ANAPC5 mutations. However, mutations in other subunits of the APC/C complex have been associated with developmental disorders, intellectual disabilities, and various types of cancer.
Depletion or mutation of ANAPC5 can lead to dysregulation of the cell cycle, genomic instability, and impaired DNA repair. This can result in various cellular defects, including aberrant mitosis, cell cycle arrest, and increased susceptibility to genetic mutations and cancer development.
Yes, ANAPC5 interacts with various proteins. Some known interacting partners include other subunits of the APC/C complex, proteins involved in DNA repair (such as BRCA1 and RAD51), transcription factors (such as E2F and p53), and proteins involved in centrosome duplication (such as CEP63 and CEP152).
ANAPC5 has been found to be involved in regulating centrosome duplication, an essential process for proper mitotic spindle formation. It interacts with proteins involved in centrosome duplication, indicating its role in ensuring the accurate replication of centrosomes during the cell cycle.
ANAPC5, through its association with the APC/C complex, can modulate DNA repair processes. It interacts with proteins involved in DNA repair, such as BRCA1 and RAD51, potentially influencing the repair of DNA damage and maintaining genomic stability.
Customer Reviews (4)
Write a reviewThey can provide valuable guidance in experimental design, optimization, and troubleshooting, helping researchers overcome challenges and maximize the efficiency of their trials.
The manufacturer's technical support can also aid researchers in the data analysis phase.
They can offer insights into interpreting results and assist in identifying trends or patterns that may be relevant to the specific research question.
the manufacturer's technical support is instrumental in assisting researchers throughout their trials.
Ask a Question for All ANAPC5 Products
Required fields are marked with *
My Review for All ANAPC5 Products
Required fields are marked with *