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Apaf1

  • Official Full Name

    APAF1 apoptotic peptidase activating factor 1

  • Overview

    Apaf-1 is a cytosolic protein that rests in a latent state until bound by cytochrome c (Apaf-2). The resulting Apaf-1/cytochrome c complex associates with the zymogen form of caspase-9 (Apaf-3) in the presence of dATP or ATP, promoting the autocatalytic activation of caspase-9. This antibody will be useful in studies of the activation of caspases through Apaf-1 and also in metastatic melanomas where Apaf-1 is inactivated which leads to defects in the execution of apoptotic cell death.
  • Synonyms

    APAF1; apoptotic peptidase activating factor 1; apoptotic peptidase activating factor , apoptotic protease activating factor; apoptotic protease-activating factor 1; APAF 1; CED4; APAF-1; DKFZp781B1145;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Antibody
  • Protein Pre-coupled Magnetic Beads
  • Human
  • Mouse
  • Rat
  • Zebrafish
  • E.coli
  • HEK293
  • Mammalian Cell
  • Wheat Germ
  • Yeast
  • GST
  • His
  • His (Fc)
  • Avi
  • His|T7
  • B2M
  • N/A
  • N
  • No tag
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human APAF1-665H Recombinant Human APAF1 protein, GST-tagged Wheat Germ GST
Human APAF1-1442H Recombinant Human APAF1 protein, His & T7-tagged E.coli His/T7 Ser31~Phe350 (Accession # O14727)
Human APAF1-89HCL Recombinant Human APAF1 cell lysate N/A
Human APAF1-27358TH Recombinant Human APAF1 Wheat Germ N/A 100 amino acids
Human APAF1-0421H Recombinant Human APAF1 Protein (Ser31-Phe350), N-His-tagged E.coli N-His Ser31-Phe350
Human APAF1-0783H Recombinant Human APAF1 Protein (M1-S97), Tag Free E.coli No tag M1-S97
Human APAF1-1329H Recombinant Human APAF1 Protein (104-415 aa), His-tagged Yeast His 104-415 aa
Human APAF1-0784H Recombinant Human APAF1 Protein (M1-S97), His tagged E.coli His M1-S97
Human APAF1-018H Recombinant Human APAF1 protein, His-B2M-tagged E.coli His-B2M 104-415aa
Mouse Apaf1-1755M Recombinant Mouse Apaf1 protein, His-tagged E.coli His Arg872~Phe1135
Mouse APAF1-1754M Recombinant Mouse APAF1 Protein Mammalian Cell His
Mouse APAF1-612M Recombinant Mouse APAF1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse APAF1-612M-B Recombinant Mouse APAF1 Protein Pre-coupled Magnetic Beads HEK293
Rat APAF1-706R Recombinant Rat APAF1 Protein Mammalian Cell His
Rat Apaf1-2062R Recombinant Rat Apoptotic Peptidase Activating Factor 1, His-tagged E.coli His
Rat Apaf1-197R Recombinant Rat Apaf1 Protein, His-tagged E.coli N-His Lys4-Gln344
Rat APAF1-362R-B Recombinant Rat APAF1 Protein Pre-coupled Magnetic Beads HEK293
Rat APAF1-362R Recombinant Rat APAF1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Zebrafish APAF1-9148Z Recombinant Zebrafish APAF1 Mammalian Cell His
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Apaf1 Related Articles
  • Apaf1 Related Signal Pathway

Apaf1 involved in several pathways and played different roles in them. We selected most pathways Apaf1 participated on our site, such as p signaling pathway, Apoptosis, Alzheimers disease, which may be useful for your reference. Also, other proteins which involved in the same pathway with Apaf1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
p signaling pathwayGADD45AA;EI24;APAF1;Casp3;CCNB2;ZMAT3;RRM2B;PPM1DB;MDM4
ApoptosisIRF2A;HIST1H1C;XIAP;PIK3R2;PIK3CG;IL1A;PIK3R1;FADD;PLEC
Alzheimers diseaseATP5H;CHP;NDUFB3;COX8A;ATP5C1;COX7C;NDUFV1;UQCRC1;BACE2
Parkinsons diseaseCOX7A2L;NDUFS5;PINK1;SNCA;COX8C;NDUFB2;UQCRC1;ATP5B;SDHB
Amyotrophic lateral sclerosis (ALS)GRIN2B;Casp3;BID;CCS;TNFRSF1A;APAF1;RAC1;MAP3K5;MAPK11
Huntingtons diseaseCOX6A2;NDUFV2;GRM5;UQCRFS1;COX7B2;BAX;POLR2H;DNALI1;NDUFA6
LegionellosisRAB1A;EEF1G;RAB1;CASP1;NAIP6;CYCS;CASP8;NFKB1;ITGAM
TuberculosisCLEC7A;TNF;FCGR2A;HLA-DRA;HLA-DMA;PPP3CB;TLR9;MAPK12;CAMK2A
Hepatitis BNFKBIA;IFIH1;IL6;IFNA16;PIK3R5;BAD;TLR3;IFNA6;PTK2B
Small cell lung cancerPIK3R3;LAMB1;RELA;CKS2;LAMB4;LAMC2;TRAF2;AKT1;CASP9

Apaf1 has several biochemical functions, for example, ADP binding, ATP binding, cysteine-type endopeptidase activator activity involved in apoptotic process. Some of the functions are cooperated with other proteins, some of the functions could acted by Apaf1 itself. We selected most functions Apaf1 had, and list some proteins which have the same functions with Apaf1. You can find most of the proteins on our site.

Function Related Protein
ADP bindingMIEF1;GLUD2;PRPS1;ABCG1;CYB5R3;CHORDC1;ATP1A1;VCP;TAP1
ATP bindingEPHA4A;MYH15;ZAK;P2RX4B;NLRP9C;RET;SNRKB;MYO1H;MLKL
cysteine-type endopeptidase activator activity involved in apoptotic processNOD1;NLRP12;PYCARD;CTSH;NLRP1;CASP1;BAD;MAPK9;APAF1
heat shock protein bindingPPP5C;BAK1;PARK2;PDXP;DNAJA3;DAXX;APAF1;LIMK1;HSPA1L
identical protein bindingLEPR;RYR2;TRIP10;NPR2;CCL4;SLK;PKD2L1;LECT2;SNCAIP
nucleotide bindingATP2B3A;PIP5K2;GLULB;CAMK2D2;SR140;PRKCBB;SREK1;IMPDH2;Fert2
protein bindingSPAT-1;RPS3;SRGN;LCMT1;SMARCAL1;STOML2;SMN1;ZBTB32;STK10

Apaf1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with Apaf1 here. Most of them are supplied by our site. Hope this information will be useful for your research of Apaf1.

CASP9

Brydges, S; Kastner, DL; et al. The systemic autoinflammatory diseases: Inborn errors of the innate immune system. CURRENT CONCEPTS IN AUTOIMMUNITY AND CHRONIC INFLAMATION 305:127-160(2006).
Gressner, O; Schilling, T; et al. TAp63 alpha induces apoptosis by activating signaling via death receptors and mitochondria. EMBO JOURNAL 24:2458-2471(2005).
  • Q&As
  • Reviews

Q&As (20)

Ask a question
What is the biological function of APAF1 ? 12/16/2022

Recombinant APAF1 protein is an important apoptosis-promoting factor that can play a key role in programmed apoptosis.

Are there any side effects of APAF1 ? 11/15/2022

APAF1 is almost non-toxic side effects, will not cause great harm to the human body.

Can APAF1 replace existing antitumor drugs? 10/18/2022

Recombinant APAF1 protein cannot completely replace existing anti-tumor drugs, but it can be used as an important part of comprehensive tumor therapy, which plays an important role in improving the therapeutic effect and reducing the toxic side effects of dose.

What is the latest progress in the clinical application of APAF1 ? 09/02/2022

The latest research shows that the APAF1 can be used as a target of novel tumor immunotherapy, and has a good effect on tumor treatment.

Does recombinant APAF1 have a role in human ER stress response? 06/21/2022

Recombinant APAF1 protein plays an important role in human endoplasmic reticulum stress response and can protect the maintenance of endoplasmic reticulum function by inhibiting apoptosis.

How to prepare APAF1 ? 01/20/2022

APAF1 can be prepared by gene recombination technology, which first expresses the target protein in a large number of expression hosts such as bacteria, and then obtains high purity APAF1 through purification technology.

What is the expression pattern of APAF1 in the human body? 12/20/2021

APAF1 is widely distributed in the human body, expressed not only in tumor cells, but also in many tissues such as heart, liver, and stomach.

What clinical tests and treatments can APAF1 be used for? 11/13/2021

APAF1 can be used in early diagnosis of tumor, prognosis of tumor, and monitoring of tumor therapeutic effect.

Can APAF1 be clearly seen in electron microscope photos? 09/08/2021

The APAF1 has a clear recognition ability for electron microscopy photos, and can be used to observe the effect of specific cells and gene transformations.

What is the relationship between APAF1 and p53 protein? 06/19/2021

APAF1 and p53 are closely related in the activation and regulation of apoptosis pathway.

Can small molecule compounds regulate the expression and activity of APAF1? 06/07/2021

Yes, small molecule compounds have regulatory effects on the expression and activity of APAF1.

What is the antigenicity of APAF1 ? 11/20/2020

APAF1 is low in antigenicity, so it is not easy to produce an immune response.

What is apoptosis resistance? Does APAF1 affect apoptosis resistance? 04/11/2020

Apoptosis resistance is a physical state in which tumor cells overcome the production of apoptotic signals. APAF1 can regulate apoptosis pathway and help to reduce apoptosis resistance.

Does APAF1 have application value in drug dose adjustment? 03/02/2020

Yes, the expression and activity level of APAF1 can be used as a standard for drug dose adjustment, which can play a regulatory role in the effect of tumor therapy.

What is the relationship between APAF1 and liver cancer and breast cancer? 12/26/2019

Recombinant APAF1 protein plays an important role in the development of liver cancer and breast cancer, and can be used as a target for early diagnosis and treatment.

What is the role of APAF1 in tumor resistance? 11/24/2019

APAF1 in tumor resistance may be affected by some factors that can lead to the weakening of apoptotic pathway processes, thus affecting the efficacy of tumor therapy.

Can APAF1 be used to study the relationship between apoptosis and tumor? 11/11/2019

Yes, the recombinant APAF1 protein can be used to study the role and mechanism of apoptosis signaling pathway in tumorigenesis and development.

Which experimental kits can APAF1 be used to prepare? 08/15/2019

APAF1 can be used in Western blot kits, ELISA kits, Western blot kits, etc.

Does APAF1 have potential applications in tumor therapy? 06/13/2019

Yes, recombinant APAF1 protein in tumor therapy can be used to develop novel targeted therapeutic drugs, such as vaccines and immune checkpoint inhibitors.

What is the position and role of APAF1 in apoptosis pathway? 05/15/2019

PAF1 can bind to other apoptotic proteins such as cytochrome c to promote the activation of Karber gene and initiate the apoptotic mechanism.

Customer Reviews (4)

Write a review
Reviews
11/11/2021

    This protein is really excellent, every time it is used, it maintains a very stable performance, which can be seen that its production process is very careful.

    10/19/2021

      Short half-life and high clearance properties of AP5Z1 make it an even greater advantage in emergency situations where rapid therapeutic effect is needed.

      04/25/2021

        Green production process of AP5Z1 is very environmentally friendly, from the selection of raw materials to the production process reflects the concept of green production, without any negative impact on the environment.

        02/13/2019

          The repeatability of AP5Z1 can be said to be unmatched, and the results are very consistent in every experiment or application, which is an important reflection of its excellent quality.

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