API5
-
Official Full Name
apoptosis inhibitor 5
-
Overview
This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. -
Synonyms
API5; apoptosis inhibitor 5; AAC 11; AAC11; API5 like 1; API5L1; fibroblast growth factor 2 interacting factor 2; migration inducing protein MIG8; FIF; antiapoptosis clone 11 protein; migration-inducing protein MIG8; cell migration-inducing gene 8 protein;
- Recombinant Proteins
- Protein Pre-coupled Magnetic Beads
- Chicken
- Human
- Mouse
- Rhesus Macaque
- Zebrafish
- E.coli
- E.Coli or Yeast
- HEK293
- HEK293T
- Mammalian Cell
- Wheat Germ
- GST
- His
- His (Fc)
- Avi
- Myc
- DDK
- MYC
- Myc|DDK
- Involved Pathway
- Protein Function
- Interacting Protein
- API5 Related Articles
API5 involved in several pathways and played different roles in them. We selected most pathways API5 participated on our site, such as , which may be useful for your reference. Also, other proteins which involved in the same pathway with API5 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
Pathway Name | Pathway Related Protein |
---|
API5 has several biochemical functions, for example, fibroblast growth factor binding, poly(A) RNA binding. Some of the functions are cooperated with other proteins, some of the functions could acted by API5 itself. We selected most functions API5 had, and list some proteins which have the same functions with API5. You can find most of the proteins on our site.
Function | Related Protein |
---|---|
fibroblast growth factor binding | FGFR4;SCN5A;Api5;GLG1B;FGFR1;FGFR2;S100A13;FGFR1A;RPS2 |
poly(A) RNA binding | TUFM;UCHL5;SRPR;CS;GNL2;RPL28;HDGF;ALG13;XRCC6 |
API5 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with API5 here. Most of them are supplied by our site. Hope this information will be useful for your research of API5.
- Q&As
- Reviews
Q&As (20)
Ask a questionThe mutation of API5 gene can be detected by PCR, sequencing, immunohistochemistry and other methods.
Recombinant API5 protein has an important clinical application prospect in prognostic diagnosis, and can provide an important basis for accurate treatment.
Recombinant API5 protein can promote the metastasis of tumor cells and further promote tumor growth and progression.
The specific adverse reactions need to be determined on a case-by-case basis. So far, studies have shown no serious adverse reactions.
Clinical trials can be conducted to evaluate the efficacy of recombinant API5 protein in the treatment of tumors.
Current studies have shown that recombinant API5 protein can play important biological functions in cancer cells and their microenvironment, and has a wide range of therapeutic prospects.
The targeting and specificity of API5 can be improved by constructing targeted drugs, probes, antibodies and other means.
Recombinant API5 protein can enhance the tolerance of tumor cells to radiotherapy by inhibiting apoptosis of tumor cells.
API5 is an inhibitor of apoptosis, which has the function of inhibiting apoptosis in cells and promoting cell survival.
Recombinant API5 proteins can bind to other apoptosis-related cytokines and proteins, thereby regulating cell survival and apoptosis signaling pathways.
Recombinant API5 protein can enhance the effect of tumor immunotherapy by inhibiting the apoptosis of immune cells.
The expression and purification of API5 can be optimized by optimizing the carrier, culture conditions, purification methods and other means.
The stability of API5 in vivo can be improved by building a stable expression system, nucleic acid drug targeting, nanotechnology and other means.
The protein encoded by the API5 gene is composed of 359 amino acids with a molecular weight of about 40kDa and contains a apoptosis inhibition domain (AID) domain.
The expression level of API5 can be detected by Western blot, immunohistochemistry, PCR and other methods.
The recombinant API5 protein can be efficiently delivered to tumor cells by means of nanotechnology, gene transfection, cell delivery, etc.
The pharmacokinetics and toxicology of API5 s can be evaluated through animal studies and in vitro studies.
The biological activity of API5 can be improved by point mutation, structural optimization and sensitivity improvement.
Recombinant API5 protein plays an important role in the occurrence, growth, metastasis and progression of a variety of tumors.
Recombinant API5 proteins can usually be prepared in E. coli or eukaryotic cells by gene recombination techniques.
Customer Reviews (3)
Write a reviewCharacteristics of short half-life and high clearance provide greater potential for recombinant proteins in the treatment of various diseases, and are expected to become a new generation of therapeutic drugs.
The stability of API5 is excellent, and its structure and function remain stable even after multiple freeze-thaw cycles, showing excellent product quality.
Western blot experiments using API5 can obtain very consistent results, indicating its high repeatability and reproducibility.
Ask a Question for All API5 Products
Required fields are marked with *
My Review for All API5 Products
Required fields are marked with *