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ARF1

  • Official Full Name

    ADP-ribosylation factor 1

  • Overview

    ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
  • Synonyms

    ARF1; ADP-ribosylation factor 1;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Chicken
  • Cynomolgus Monkey
  • Human
  • Mouse
  • Rat
  • Rhesus Macaque
  • Zebrafish
  • E. coli
  • E.coli
  • E.Coli or Yeast
  • HEK293
  • HEK293T
  • In Vitro Cell Free System
  • Mammalian Cell
  • Wheat Germ
  • GST
  • His
  • His (Fc)
  • Avi
  • Myc
  • DDK
  • MYC
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human ARF1-2769H Recombinant Human ADP-ribosylation Factor 1, His-tagged E.coli His
Human ARF1-9799H Recombinant Human ARF1, GST-tagged E.coli GST 1-181a.a.
Human ARF1-744H Recombinant Human ARF1 protein, GST-tagged Wheat Germ GST
Human ARF1-224H Recombinant Human ARF1 protein(Met1-Lys181), His-tagged E. coli N-His Met1-Lys181
Human ARF1-8760HCL Recombinant Human ARF1 293 Cell Lysate HEK293 N/A
Human ARF1-8761HCL Recombinant Human ARF1 293 Cell Lysate HEK293 N/A
Human ARF1-1938H Recombinant Human ARF1 Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human ARF1-2544H Recombinant Human ARF1 protein, His-GST & Myc-tagged E.coli His-GST & Myc 2-181aa
Human ARF1-506H Recombinant Human ARF1 Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human ARF1-1165HF Recombinant Full Length Human ARF1 Protein, GST-tagged In Vitro Cell Free System GST 181 amino acids
Mouse ARF1-1837M Recombinant Mouse ARF1 Protein Mammalian Cell His
Mouse ARF1-658M Recombinant Mouse ARF1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse ARF1-658M-B Recombinant Mouse ARF1 Protein Pre-coupled Magnetic Beads HEK293
Mouse Arf1-634M Recombinant Mouse Arf1 Protein, MYC/DDK-tagged HEK293T MYC/DDK
Rat ARF1-747R Recombinant Rat ARF1 Protein Mammalian Cell His
Rat Arf1-3205R Recombinant Rat Arf1, His-tagged E.Coli or Yeast His 181
Rat ARF1-403R-B Recombinant Rat ARF1 Protein Pre-coupled Magnetic Beads HEK293
Rat ARF1-403R Recombinant Rat ARF1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Cynomolgus Monkey ARF1-308C Recombinant Cynomolgus ARF1 Protein, His-tagged Mammalian Cell His
Cynomolgus Monkey ARF1-58C Recombinant Cynomolgus Monkey ARF1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Cynomolgus Monkey ARF1-58C-B Recombinant Cynomolgus Monkey ARF1 Protein Pre-coupled Magnetic Beads HEK293
Rhesus Macaque ARF1-378R Recombinant Rhesus monkey ARF1 Protein, His-tagged Mammalian Cell His
Rhesus Macaque ARF1-207R-B Recombinant Rhesus Macaque ARF1 Protein Pre-coupled Magnetic Beads HEK293
Rhesus Macaque ARF1-207R Recombinant Rhesus Macaque ARF1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Zebrafish ARF1-11499Z Recombinant Zebrafish ARF1 Mammalian Cell His
Chicken ARF1-1496C Recombinant Chicken ARF1 Mammalian Cell His
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • ARF1 Related Articles
  • ARF1 Related Signal Pathway

ARF1 involved in several pathways and played different roles in them. We selected most pathways ARF1 participated on our site, such as Adaptive Immune System, Arf1 pathway, Arf6 downstream pathway, which may be useful for your reference. Also, other proteins which involved in the same pathway with ARF1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Adaptive Immune SystemCLEC2B;CTSLL;LAIR1;CTSD;MKRN1;RNF34;SH2D1B;PIK3AP1;OSCAR
Arf1 pathwayAP2M1;ARFIP2;CLTB;ARF1;CLTA;USO1;AP2A1
Arf6 downstream pathway
COPI Mediated TransportARCN1;ARCN1A;ARF1;COPB2;COPB1;TERFA;COPE;COPG;COPZ1
Cell cycleMND1;HIST3H2BB;YWHAZ;USO1;MSH5;CEP135;TGFB1A;NEK7;CCP110
Class I PI3K signaling eventsADAP1;ARF1;PLEKHA1;ITK;BLK
Clathrin derived vesicle buddingHIP1RB;AP1B1;DTNBP1;SNAPIN;PICALMB;CTSZ;TCEAL4;CLTA;GAK
DiseaseLRRFIP1;TIRAP;TTR;EEF2;NMT1;AP2A1;DOCK2;GYG1;GYG2

ARF1 has several biochemical functions, for example, GDP binding, GTP binding, GTPase activity. Some of the functions are cooperated with other proteins, some of the functions could acted by ARF1 itself. We selected most functions ARF1 had, and list some proteins which have the same functions with ARF1. You can find most of the proteins on our site.

Function Related Protein
GDP bindingPRPS1;RAN;RAP1B;IIGP1;RAB22A;RAB17;RRAS;RAB21;RAB8A
GTP bindingMXA;IRGM;IFT22;GMPPAB;SEPT5B;GNAZ;GTPBP3;ARL15;IRGC
GTPase activityGNB5;IIGP1;GUF1;RAB26;GNAO1A;GNA11B;ATL1;GFM2;RAB8A
magnesium ion bindingPRKACB;PDXK;ENO2;PI4K2A;HPGDS;COMT;CIB4;PPA2;NCS1
phospholipase D activator activity
poly(A) RNA bindingLUC7L3;SFPQ;DDX52;U2AF2;DDX3X;TRMT1;TPR;RPS15;ACTN4
protein bindingUSH1G;ARAP1;TBC1D10A;SNX7;BTN3A1;FGFR4;DNAJB2;KISS1;DCTN1
receptor signaling protein activitySMAD1;FCGR1A;GNAZ;DOK2;DOK5;ARF1;ADRB1;RGS14A;NGF

ARF1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with ARF1 here. Most of them are supplied by our site. Hope this information will be useful for your research of ARF1.

CYTH2; 1-phosphatidyl-1d-myo-inositol 3,5-bisphosphate; 1-phosphatidyl-1d-myo-inositol 4,5-bisphosphate; SPAG9; ASAP1; GEA1; Asap1; Asap1; Kif1c; MYC; Bach1; AP1B1; Proser1

Richards, AL; Soares-Martins, JAP; et al. Generation of Unique Poliovirus RNA Replication Organelles. MBIO 5:-(2014).
Hehnly, H; Xu, WD; et al. Cdc42 Regulates Microtubule-Dependent Golgi Positioning. TRAFFIC 11:1067-1078(2010).
  • Q&As
  • Reviews

Q&As (21)

Ask a question
Can ARF1 be regulated by post-translational modifications? 03/12/2023

Yes, ARF1 can undergo various post-translational modifications, including phosphorylation, acetylation, and lipidation. These modifications can affect its activity, localization, and interactions with other proteins, providing a means of regulation and modulation in response to cellular cues and signaling events.

How is ARF1 protein implicated in cancer? 02/11/2023

Dysregulation of ARF1 has been observed in some cancers, where it can contribute to abnormal membrane trafficking and facilitate tumor cell proliferation, invasion, and metastasis. Targeting ARF1 and its associated pathways may hold therapeutic potential in cancer treatment.

What cellular processes are regulated by ARF1 protein? 04/06/2022

ARF1 regulates membrane trafficking pathways, including protein sorting, vesicle formation, and transport between organelles such as the Golgi apparatus, plasma membrane, and endosomes.

Can ARF1 play a role in neuronal processes? 10/16/2021

Yes, ARF1 is involved in various neuronal processes, including axonal development, synapse formation, and neurotransmitter release. It regulates the trafficking and recycling of synaptic vesicles, ensuring the proper function of neuronal communication.

Can ARF1 protein be targeted for therapeutic purposes? 10/10/2021

Yes, ARF1 and its regulators are potential targets for therapeutic intervention in diseases involving abnormal membrane trafficking, such as cancer and neurodegenerative disorders. Modulating ARF1 activity could potentially impact these processes.

Are there any known diseases caused by ARF1 mutations? 07/27/2021

Although rare, mutations in ARF1 have been associated with certain genetic disorders. For example, mutations in ARF1 have been linked to arthrogryposis, renal dysfunction, and cholestasis syndrome (ARC) characterized by multisystemic impairments.

Can ARF1 protein be regulated by signaling pathways? 06/13/2021

Yes, ARF1 activity can be modulated by various signaling pathways. For example, certain protein kinases can phosphorylate ARF1, leading to its activation or inactivation, depending on the context.

What are the regulators of ARF1 activation? 10/25/2020

ARF1 activation is regulated by guanine nucleotide exchange factors (GEFs) that facilitate the exchange of GDP for GTP, and GTPase-activating proteins (GAPs) that promote GTP hydrolysis, inactivating ARF1.

How is ARF1 involved in membrane recycling? 10/19/2019

ARF1 is essential for the recycling of membranes and receptors within the cell. It regulates the formation of endosomal compartments and mediates the sorting and retrieval of cargo molecules from endosomes back to the plasma membrane or other intracellular compartments.

Are there any diseases associated with abnormalities in ARF1 protein? 04/22/2019

Yes, mutations or dysregulation of ARF1 have been implicated in various diseases, including certain types of cancer, neurodegenerative disorders, and metabolic diseases.

Are there any drugs or compounds that specifically target ARF1? 04/18/2019

Currently, there are no drugs specifically designed to target ARF1. However, certain compounds, such as brefeldin A, have been shown to inhibit ARF1 function by disrupting its activation and guanine nucleotide exchange factors (GEFs). These compounds have been used as research tools to study ARF1 function and vesicular trafficking in cellular processes.

How does ARF1 protein regulate cargo sorting? 01/24/2019

ARF1 interacts with adaptor proteins, such as AP-1 and AP-3, to facilitate the recognition and targeting of specific cargo molecules. This ensures that the appropriate cargo is packaged into vesicles for transport to their intended destinations.

Can ARF1 be regulated by lipid signaling? 09/22/2018

Yes, ARF1 can be regulated by lipid signaling pathways. Certain lipids, such as phosphoinositides, can directly bind and modulate the activity of ARF1 and its associated factors, influencing vesicle formation and cargo sorting.

How is ARF1 protein involved in endocytosis? 07/19/2018

ARF1 is involved in clathrin-mediated endocytosis, a process in which extracellular molecules are internalized by cells. It helps regulate the formation of clathrin-coated pits, which are responsible for the uptake of specific cargo molecules.

How is ARF1 protein involved in protein trafficking within the Golgi apparatus? 06/04/2018

ARF1 plays a central role in the maintenance of Golgi structure and function. It promotes the recruitment of coat proteins to specific Golgi compartments, enabling the formation of transport vesicles and the sorting of proteins for proper secretion or delivery to other organelles.

Does ARF1 protein have any post-translational modifications? 03/02/2017

Yes, like many other proteins, ARF1 undergoes post-translational modifications. One common modification is lipidation, where it is covalently attached to a lipid moiety, which helps anchor it to specific membrane compartments.

Does ARF1 have any interactions with the cytoskeleton? 09/25/2016

Yes, ARF1 has been shown to interact with components of the cytoskeleton, such as actin and microtubules. These interactions play a role in vesicle trafficking and membrane remodeling processes, allowing for the movement of vesicles along the cytoskeletal network.

Are there any known interacting partners of ARF1 protein? 07/19/2016

ARF1 interacts with various effector proteins, such as coatomer protein complex I (COPI), clathrin, and adaptor proteins like AP-1 and AP-3, which mediate vesicle formation and cargo selection.

How is ARF1 protein involved in vesicle formation? 05/15/2016

ARF1 plays a crucial role in the formation of coated vesicles. It recruits coat proteins, such as COPI and clathrin, to specific membranes, initiating the budding process and ultimately leading to the formation of vesicles.

How is ARF1 involved in lipid metabolism? 03/13/2016

ARF1 plays a role in lipid metabolism by regulating the transport and sorting of lipids within cells. It helps control the intracellular trafficking of lipid droplets, contributes to the formation of lipid rafts, and influences the activity of lipid-modifying enzymes, impacting lipid homeostasis and metabolism.

Can ARF1 protein interact with other small GTPases? 01/02/2016

Yes, ARF1 can interact with other small GTPases, such as Rho and Rab proteins, to coordinate and regulate various cellular processes. These interactions provide a means of crosstalk and integration between different signaling pathways.

Customer Reviews (8)

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Reviews
12/01/2021

    The manufacturer's support has played a vital role in my work, providing expert guidance and troubleshooting assistance whenever needed.

    11/12/2021

      Its reliable bioactivity makes it an ideal choice for a diverse range of experimental applications.

      06/09/2020

        Its reliability, specificity, and consistent performance enable me to obtain accurate data to further my research goals.

        04/17/2019

          The manufacturer offers exceptional technical assistance, guiding researchers through various stages of their experiments.

          09/04/2018

            By providing comprehensive data and resources, the manufacturer enables researchers to design and implement their trials with confidence, ensuring the desired outcomes.

            07/27/2017

              the ARF1 protein offers exceptional advantages in my trials, facilitating my exploration of important cellular mechanisms.

              02/09/2017

                This information is crucial for researchers in understanding the protein's behavior and interaction within their specific experimental context.

                10/14/2016

                  I am grateful for their dedication and commitment to ensuring the success of my experiments.

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