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MS4A1

  • Official Full Name

    membrane-spanning 4-domains, subfamily A, member 1

  • Overview

    This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
  • Synonyms

    MS4A1; membrane-spanning 4-domains, subfamily A, member 1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16; B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Canine
  • Canis lupus familiaris (Dog) (Canis familiaris)
  • Cynomolgus
  • Cynomolgus Monkey
  • Dog
  • Ferret
  • Homo sapiens (Human)
  • Human
  • Monkey
  • Mouse
  • Mus musculus (Mouse)
  • Baculovirus-Insect cells
  • E. coli
  • E.coli
  • E.coli expression system
  • HEK293
  • HEK293T
  • Human Cell
  • In vitro E. coli expression system
  • Insect Cell
  • Mammalian Cell
  • Mammalian cells
  • Wheat Germ
  • Yeast
  • Biotin|Fc
  • Fc
  • Flag
  • Flag,8His
  • GFP
  • GST
  • His
  • His (Fc)
  • Avi
  • His|GST
  • His|SUMO
  • His|trxA
  • Myc
  • DDK
  • MYC
  • Myc|DDK
  • N/A
  • N
  • 8×His
  • TEV
  • Trx
  • TrxA
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human MS4A1-17H Active Recombinant Human MS4A1 protein, Fc-tagged HEK293 Fc Ile 141 – Tyr 184
Human MS4A1-19H Active Recombinant Human MS4A1 Protein, TrxA-tagged E.coli TrxA
Human MS4A1-17HAF488 Active Recombinant Human MS4A1 Protein, Fc-tagged, Alexa Fluor 488 conjugated HEK293 Fc Ile141-Tyr184
Human MS4A1-470H Active Recombinant Human MS4A1 Protein (Ile141-Ser188), TrxA-tagged, Biotinylated E.coli TrxA Ile141-Ser188
Human MS4A1-17HAF647 Active Recombinant Human MS4A1 Protein, Fc-tagged, Alexa Fluor 647 conjugated HEK293 Fc Ile141-Tyr184
Human MS4A1-17HAF555 Active Recombinant Human MS4A1 Protein, Fc-tagged, Alexa Fluor 555 conjugated HEK293 Fc Ile141-Tyr184
Human MS4A1-151H Active Recombinant Human MS4A1 protein, Flag-tagged HEK293 Flag Met1-Pro297
Human MS4A1-17HF Active Recombinant Human MS4A1 Protein, Fc-tagged, FITC conjugated HEK293 Fc Ile141-Tyr184
Human MS4A1-11H Recombinant Human MS4A1 Wheat Germ N/A
Human MS4A1-16H Recombinant Human MS4A1, His-tagged E.coli His
Human MS4A1-10H Recombinant Human MS4A1, GST-tagged Wheat Germ GST
Human MS4A1-8530H Recombinant Human MS4A1, His-tagged Human Cell His
Human MS4A1-826H Recombinant Human MS4A1, Biotin-Fc-tagged HEK293 Biotin/Fc
Human MS4A1-18H Recombinant Human MS4A1 protein, His/trxA-tagged E.coli His/trxA Ile 141 - Tyr 184
Human MS4A1-22H Recombinant Human MS4A1 Protein (T2-P297 end), N-8×His-Flag-TEV-tagged Insect Cell N-8×His-Flag-TEV T2-P297 end
Human MS4A1-1542HCL Recombinant Human MS4A1 cell lysate Human Cell N/A
Human MS4A1-7308HAF555 Recombinant Human MS4A1 Protein, His-tagged, Alexa Fluor 555 conjugated His
Human MS4A1-1933H Recombinant Human MS4A1 Protein, MYC/DDK-tagged HEK293 Myc/DDK
Human MS4A1-17HP Recombinant Human MS4A1 protein, Fc-tagged, R-PE labeled HEK293 Fc
Human MS4A1-3928H Active Recombinant Human MS4A1 Full Length Transmembrane protein, His-tagged(VLPs) HEK293 His 1-297aa
Human MS4A1-3929H Recombinant Human MS4A1 full length protein, His-tagged(Nanodisc) HEK293 His Met 1 - Pro 297
Human MS4A1-48392H Active Recombinant Human MS4A1 protein, His-tagged Baculovirus-Insect cells His Met1-Pro297
Human MS4A1-17HA Recombinant Human MS4A1 protein, Fc-tagged, APC labeled HEK293 Fc
Human MS4A1-420H Recombinant Human MS4A1 protein, His-Avi-tagged E. coli His-Avi It contains Ile141-Ser188.
Human MS4A1-3932H Recombinant Human MS4A1 full length protein, His/Avi-tagged, Biotinylated(Nanodisc) HEK293 His/Avi Met 1 - Pro 297
Human MS4A1-7308HAF647 Recombinant Human MS4A1 Protein, His-tagged, Alexa Fluor 647 conjugated His
Human MS4A1-1135H Recombinant Human MS4A1 Protein, His&GST-tagged E.coli N-His&GST Lys142-Pro297
Human MS4A1-3930H Active Recombinant Human MS4A1 protein HEK293 N/A Met 1 - Pro 297
Human MS4A1-391H Active Recombinant Fluorescent Human MS4A1 Full Length protein(VLPs), GFP-tagged HEK293 GFP Met 1 - Pro 297
Human MS4A1-7308HF Recombinant Human MS4A1 Protein, His-tagged, FITC conjugated His
Human MS4A1-5471H Recombinant Human MS4A1 protein, His-tagged Yeast His 141-297aa
Human MS4A1-3931H Active Recombinant Human MS4A1 protein, His-Avi-tagged, Biotinylated HEK293 His-Avi Met 1 - Pro 297
Human MS4A1-1439H Recombinant Human MS4A1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human MS4A1-1649H Recombinant Human MS4A1 Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human MS4A1-118HFL Recombinant Full Length Human MS4A1 Protein, C-Flag-tagged Mammalian cells Flag
Human MS4A1-7308HAF488 Recombinant Human MS4A1 Protein, His-tagged, Alexa Fluor 488 conjugated His
Human MS4A1-23H Recombinant Human MS4A1 Protein (T2-P297 end), N-8×His-Flag-TEV-tagged Insect Cell N-8×His-Flag-TEV T2-P297 end
Human MS4A1-2359H Active Recombinant Human MS4A1 protein, His-Flag-tagged HEK293 His-Flag Met1-Pro297
Human MS4A1-0157H Recombinant Human MS4A1 Protein (T2-P297 end), Flag, 8×His tagged Insect Cell Flag,8His T2-P297 end
Human MS4A1-0767H Recombinant Human MS4A1 Protein (Ile143-Ser188), N-His and N-Trx tagged E.coli N-His and N-Trx Ile143-Ser188
Human MS4A1-1439H-B Recombinant Human MS4A1 Protein Pre-coupled Magnetic Beads HEK293
Human MS4A1-0123H Recombinant Human MS4A1 Full Length Transmembrane protein, His-tagged In vitro E. coli expression system His 1-297aa
Human MS4A1-5318H Recombinant Human MS4A1 Protein (Glu213-Pro297), N-His tagged Mammalian cells N-His Glu213-Pro297
Human MS4A1-5319H Recombinant Human MS4A1 Protein (Met1-Pro297), His tagged E.coli His Met1-Pro297
Mouse Ms4a1-4893M Recombinant Mouse Ms4a1 protein, His/SUMO-tagged E.coli His/SUMO 132-291aa
Mouse MS4A1-10113M Recombinant Mouse MS4A1 Protein Mammalian Cell His
Mouse Ms4a1-4896M Recombinant Mouse Ms4a1 protein, His-tagged Yeast His 111-291aa
Mouse Ms4a1-4899M Recombinant Mouse Ms4a1 protein, His/GST-tagged E.coli His/GST
Mouse MS4A1-5732M-B Recombinant Mouse MS4A1 Protein Pre-coupled Magnetic Beads HEK293
Mouse Ms4a1-830M Recombinant Mouse Ms4a1 Protein, MYC/DDK-tagged HEK293T MYC/DDK
Mouse MS4A1-5732M Recombinant Mouse MS4A1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse Ms4a1-5472M Recombinant Mouse Ms4a1 protein, His-tagged Yeast His 132-291aa
Mouse Ms4a1-1136M Recombinant Mouse Ms4a1 Protein, His-tagged E.coli N-His Leu133-Pro291
Monkey MS4A1-62CAF488 Recombinant Cynomolgus MS4A1 Protein, His-tagged, Alexa Fluor 488 conjugated HEK293 His 96
Monkey MS4A1-62CAF647 Recombinant Cynomolgus MS4A1 Protein, His-tagged, Alexa Fluor 647 conjugated HEK293 His 96
Monkey MS4A1-62CAF555 Recombinant Cynomolgus MS4A1 Protein, His-tagged, Alexa Fluor 555 conjugated HEK293 His 96
Monkey MS4A1-62CF Recombinant Cynomolgus MS4A1 Protein, His-tagged, FITC conjugated HEK293 His 96
Dog MS4A1-6579D Active Recombinant Dog MS4A1 protein, His-tagged(Nanodisc) HEK293 His Met 1 - Pro 297
Dog MS4A1-6578D Active Recombinant Dog MS4A1 Full Length Transmembrane protein, His-tagged(VLPs) HEK293 His 1-297aa
Dog MS4A1-1934D Recombinant Dog MS4A1 Full Length Transmembrane protein, His-tagged In vitro E. coli expression system His 1-297aa
Canine MS4A1-3048C Active Recombinant Canine MS4A1 protein, His-tagged HEK293 His Met 1-Pro 297
Canine MS4A1-3049C Active Recombinant Canine MS4A1 protein, His-tagged HEK293 His Met 1-Pro 297
Canis lupus familiaris (Dog) (Canis familiaris) RFL16853CF Recombinant Full Length Dog B-Lymphocyte Antigen Cd20(Ms4A1) Protein, His-Tagged E.coli expression system His Full Length (1-297)
Cynomolgus MS4A1-708C Recombinant Cynomolgus MS4A1 protein, His-tagged HEK293 His Glu213-Pro297
Cynomolgus MS4A1-3050C Active Recombinant Cynomolgus MS4A1 Full Length Transmembrane protein, His-tagged(VLPs) HEK293 His 1-297aa
Cynomolgus Monkey MS4A1-62C Recombinant Cynomolgus MS4A1, His tagged Human Cell Fc
Cynomolgus Monkey MS4A1-819CCL Recombinant Cynomolgus MS4A1 cell lysate Human Cell N/A
Ferret MS4A1-09F Recombinant Ferret MS4A1, His tagged Human Cell His
Ferret MS4A1-1848FCL Recombinant Ferret MS4A1 cell lysate Human Cell N/A
Ferret MS4A1-09FAF488 Recombinant Ferret MS4A1 Protein, His-tagged, Alexa Fluor 488 conjugated HEK293 His 96
Ferret MS4A1-09FAF555 Recombinant Ferret MS4A1 Protein, His-tagged, Alexa Fluor 555 conjugated HEK293 His 96
Ferret MS4A1-09FAF647 Recombinant Ferret MS4A1 Protein, His-tagged, Alexa Fluor 647 conjugated HEK293 His 96
Ferret MS4A1-09FF Recombinant Ferret MS4A1 Protein, His-tagged, FITC conjugated HEK293 His 96
Homo sapiens (Human) RFL14830HF Recombinant Full Length Human B-Lymphocyte Antigen Cd20(Ms4A1)-Vlps (Active) Protein, His-Tagged E.coli expression system His Full Length (1-297aa)
Homo sapiens (Human) RFL31628HF Recombinant Full Length Human B-Lymphocyte Antigen Cd20(Ms4A1) Protein, His-Tagged E.coli expression system His Full Length (1-297)
Mus musculus (Mouse) RFL3192MF Recombinant Full Length Mouse B-Lymphocyte Antigen Cd20(Ms4A1) Protein, His-Tagged E.coli expression system His Full Length (1-291)
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • MS4A1 Related Articles
  • MS4A1 Related Research Area

What is MS4A1 protein?

MS4A1 (membrane spanning 4-domains A1) gene is a protein coding gene which situated on the long arm of chromosome 11 at locus 11q12. This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. The MS4A1 protein is consisted of 297 amino acids and its molecular mass is approximately 33.1 kDa.

What is the function of MS4A1 protein?

MS4A1 is a b-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. It also acts as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.

MS4A1 Related Signaling Pathway

The MS4A1 protein is a cell membrane protein that is involved in a variety of signaling pathways and biological processes. The following are some of the signaling pathways associated with MS4A1 protein: MS4A1 protein is widely expressed in immune cells, including lymphocytes, dendritic cells, etc. It is involved in a variety of immunomodulatory processes, regulating the function of immune cells and immune responses by interacting with other signaling molecules. The MS4A1 protein is expressed in several cell types, including tumor cells and nerve cells. It plays an important role in cell proliferation and survival through PI3K-Akt signaling pathway, MAPK signaling pathway and so on.

MS4A1 Related Diseases

MS4A1, also known as CD20, protein plays an important role in the activation and differentiation of T cells, so its abnormal function may lead to immune deficiency diseases, such as congenital immune deficiency syndrome (CIDS). Its abnormal function may lead to the occurrence of autoimmune diseases, such as rheumatoid arthritis, systemic lupus erythematosus and so on. MS4A1 protein is abnormally expressed in many cancers, such as breast cancer, colon cancer, lung cancer and Non-Hodgkin's lymphoma, etc.

MS4A1-9.jpg

Fig1. A higher power view (Patient 1) shows epidermotropic lymphocytes (A), with expression of CD3 (B, D) and CD20 (C, D). Co-expression of CD3 and CD20 is confirmed on a dual CD3/CD20 immunohistochemical study (D; CD3 in red, CD20 in brown). (Kelly L Harms, 2014)

Bioapplications of MS4A1

Using the specific expression of MS4A1, researchers are exploring the possibility of wrapping drugs in specific vectors for targeted delivery by binding to MS4A1. Drugs that interact with MS4A1 are being developed for use in tumor therapy.

High Purity

High Bioactivity

MS4A1-2.jpg

Fig2. Activity Data. (MS4A1-3928H)

Case study 1: Martijn Vlaming, 2021

The presence of T cells that are dimly positive for the B cell marker CD20 is well-established in autoimmunity and correlates with disease severity in various diseases. CD20-positive T cells were previously identified as IFN-gamma producing, low proliferative, CD8 cytotoxic T cells with an effector memory (EM) differentiation state. However, the exact phenotype and relevance of CD20-positive T cells remains unclear.

To gain insight into potential functional properties of CD20 expression in T cells, CD20 was ectopically expressed on healthy human T cells and phenotypic, functional, migratory and adhesive properties were determined in vitro and in vivo. Together, these assays revealed a reduced transmigration and an enhanced adhesive profile combined with an enhanced activation status for CD20-positive T cells.

MS4A1-3.jpg

Fig1. 1.0 × 10^6 primary T cells were transduced with the CD20 or SFCMM-3 retroviral vector (mean of 50% CD20 + or 50% NGFR-positive) and the influence of CD20 expression on the viability of cells was investigated by Annexin-V and PI staining at 7 days post transduction.

MS4A1-4.jpg

Fig2. Immunohistochemical evaluation was performed in mice injected with T cells ectopically expressing CD20.

Case study 2: Veronika Kozlova, 2020

Surface protein CD20 serves as the critical target of immunotherapy in various B-cell malignancies for decades, however its biological function and regulation remain largely elusive. Using CRISPR/Cas9 technique, the researchers have abrogated CD20 expression in five different malignant B-cell lines. The findings show that CD20 deletion has no effect upon B-cell receptor signaling or calcium flux. Also B-cell survival and proliferation is unaffected in the absence of CD20. Mechanistically, we could identify a reduction in chemokine-triggered PYK2 activation, a calcium-activated signaling protein involved in activation of MAP kinases and cytoskeleton regulation. These cellular defects in consequence result in a severely disturbed homing of B cells in vivo.

MS4A1-5.jpg

Fig3. Ramos control and CD20 knockout cells were stimulated with Rituximab (RTX) or anti-IgM antibodies for indicated times (minutes).

MS4A1-6.jpg

Fig4. Defective chemokine signaling in the absence of CD20. Ramos (left panels) and MEC1 (right panels) control and CD20-knockout cells were stimulated with chemokines SDF1α or CCL19, respectively, for indicated times (minutes).

MS4A1-7.jpg

Fig1. Hematopoietic Stem Cell Differentiation Pathways & Lineage-specific Markers from R&D

MS4A1-8.jpg

Fig2. Different effector mechanisms of type I and II anti-CD20 mAbs. Type I mAbs are able to engage CDC and ADCC and cause modulation in certain B-cell malignancies but do not elicit efficient direct cell death, whereas type II mAbs can mediate direct cell death through a lysosomal pathway and engage ADCC but do not promote CDC or CD20 modulation. (Stephen A Beers, 2010)

MS4A1 involved in several pathways and played different roles in them. We selected most pathways MS4A1 participated on our site, such as Hematopoietic cell lineage, which may be useful for your reference. Also, other proteins which involved in the same pathway with MS4A1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Hematopoietic cell lineageCD1B;CD8B1;CD9;ITGA5;CD8B;IL5RA;IL3;CD1D2;CD59

MS4A1 has several biochemical functions, for example, MHC class II protein complex binding, epidermal growth factor receptor binding, protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by MS4A1 itself. We selected most functions MS4A1 had, and list some proteins which have the same functions with MS4A1. You can find most of the proteins on our site.

Function Related Protein
MHC class II protein complex bindingHSP90AB1;PKM;MS4A1;HLA-DRB1;HLA-DOA;HLA-DRA;CD74;HSPA8;HLA-DOB
epidermal growth factor receptor bindingEGF;EFEMP1;VAV2;CBLC;HBEGF;ATXN2;BTC;EPGN;ARF4
protein bindingNCF1;TCF25;PTPN12;VAMP7;CEP120;DNPEP;NDUFS7;TARSL2;DUSP5

MS4A1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with MS4A1 here. Most of them are supplied by our site. Hope this information will be useful for your research of MS4A1.

CCDC155; IGHM; IGHD; CREB3; xthA; tyrS

Ai, J; Advani, A; et al. Current status of antibody therapy in ALL. BRITISH JOURNAL OF HAEMATOLOGY 168:471-480(2015).
Winiarska, M; Bojarczuk, K; et al. Inhibitors of SRC kinases impair antitumor activity of anti-CD20 monoclonal antibodies. MABS 6:1300-1313(2014).
  • Q&As
  • Reviews

Q&As (7)

Ask a question
How is the expression of MS4A1 regulated during B-cell development and differentiation? 09/06/2022

 MS4A1 expression is tightly regulated during different stages of B-cell development. It is absent in early B-cell progenitors and gradually increases during maturation. MS4A1 expression is upregulated upon B-cell activation and can be influenced by various cytokines and signaling pathways. This tightly controlled expression pattern ensures proper B-cell development, while aberrant regulation may contribute to B-cell disorders or malignancies.

Can MS4A1 be used as a diagnostic or prognostic marker in B-cell disorders? 08/08/2022

MS4A1 expression levels have been explored as potential diagnostic or prognostic markers for certain B-cell disorders. Quantification of CD20 expression by immunohistochemistry or flow cytometry can aid in the diagnosis and classification of B-cell malignancies. Additionally, low CD20 expression levels have been associated with resistance to CD20-targeted therapies, serving as a prognostic indicator in B-cell lymphomas.

Are there any therapeutic strategies targeting MS4A1 beyond CD20-directed antibodies? 12/19/2020

While CD20-targeted antibodies have revolutionized the treatment of B-cell malignancies, further therapeutic strategies are being explored. These include enhancing CD20 internalization, using antibody-drug conjugates, and developing novel targeted therapies based on the signaling pathways associated with MS4A1. Investigating these approaches may provide alternative treatments and improve outcomes for patients with B-cell disorders.

How does MS4A1 contribute to the regulation of immune tolerance and autoimmunity? 09/30/2019

 MS4A1 helps maintain immune tolerance by preventing the production of autoreactive B cells. It contributes to the elimination of self-reactive B cell clones through mechanisms such as antibody-dependent cellular cytotoxicity and antibody-mediated phagocytosis. MS4A1 dysregulation, however, can lead to the production of autoantibodies and the development of autoimmune diseases. Understanding the fine-tuned regulation of MS4A1's functions is essential for maintaining immune homeostasis and preventing autoimmunity.

What are the interacting partners and downstream signaling pathways of MS4A1? 11/16/2017

MS4A1 interacts with multiple proteins, such as tetraspanins, Src-family kinases, and lipid rafts, which form a complex network involved in B-cell signaling. Upon ligand binding, MS4A1 activates intracellular signaling pathways, including PI3K/Akt and MAPK/ERK, which regulate B-cell survival, proliferation, and differentiation. Understanding these pathways and their crosstalk with MS4A1 can provide insights into B-cell activation and potential therapeutic targets.

What is the structure and function of the MS4A1 protein in immune cells? 11/06/2017

The MS4A1 protein, also known as CD20, is a tetraspanin membrane protein found predominantly in B cells. It plays a crucial role in B-cell development, activation, and antibody production. Structurally, CD20 consists of four transmembrane domains and a large extracellular loop. Functionally, it regulates B-cell signaling by promoting calcium influx upon B-cell receptor activation, modulating cell cycle progression, and facilitating antibody-mediated cytotoxicity.

How does MS4A1 contribute to the pathogenesis of B-cell malignancies? 09/14/2017

MS4A1 dysregulation has been implicated in B-cell malignancies, including non-Hodgkin lymphomas and leukemia. Increased expression or altered signaling of MS4A1 may promote survival and proliferation of malignant B cells. CD20-targeted therapies, such as monoclonal antibodies, have been successfully employed in treating B-cell malignancies, highlighting the significance of MS4A1 as a therapeutic target in these diseases.

Customer Reviews (3)

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Reviews
11/02/2019

    Its strong affinity allows for robust binding to target proteins, thereby enhancing the accuracy of analysis.

    04/05/2017

      Surpassing expectations, it significantly amplifies the results of my research.

      02/07/2017

        The operational guidelines for this reagent are readily comprehensible and accessible, facilitating seamless implementation of experiments, even for individuals lacking prior expertise.

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