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MYL2

  • Official Full Name

    Myosin regulatory light chain 2

  • Overview

    Myosins comprise a family of ATP-dependent motor proteins and are best known for their role in muscle contraction and their involvement in a wide range of other eukaryotic motility processes. They are responsible for actin-based motility. The term was originally used to describe a group of similar ATPases found in striated and smooth muscle cells. Following the discovery by Pollard and Korn of enzymes with myosin-like function in Acanthamoeba castellanii, a large number of divergent myosin genes have been discovered throughout eukaryotes. Thus, although myosin was originally thought to be restricted to muscle cells (hence, "myo"), there is no single "myosin" but rather a huge superfamily of genes whose protein products share the basic properties of actin binding, ATP hydrolysis (ATPase enzyme activity), and force transduction. Virtually all eukaryotic cells contain myosin isoforms. Some isoforms have specialized functions in certain cell types (such as muscle), while other isoforms are ubiquitous. The structure and function of myosin is strongly conserved across species, to the extent that rabbit muscle myosin II will bind to actin from an amoeba.
  • Synonyms

    Myosin regulatory light chain 2; MYL2;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Chicken
  • Human
  • Mouse
  • Rat
  • E.coli
  • HEK293
  • HEK293T
  • In Vitro Cell Free System
  • Mammalian Cell
  • Mammalian cells
  • Wheat Germ
  • Flag
  • GST
  • His
  • His (Fc)
  • Avi
  • Myc
  • DDK
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human MYL2-235H Active Recombinant Human Myosin, Light Chain 2, Regulatory, Cardiac, Slow E.coli N/A
Human MYL2-28864TH Recombinant Human MYL2, His-tagged E.coli His
Human MYL2-27837TH Recombinant Human MYL2, His-tagged E.coli His 166 amino acids
Human MYL2-388H Recombinant Human Slow Cardiac Myosin Regulatory light chain 2, His-tagged E.coli His
Human MYL2-4028HCL Recombinant Human MYL2 293 Cell Lysate HEK293 N/A
Human MYL2-5808H Recombinant Human MYL2 Protein, GST-tagged Wheat Germ GST
Human MYL2-1467H Recombinant Human MYL2 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human MYL2-960HFL Recombinant Full Length Human MYL2 Protein, C-Flag-tagged Mammalian cells Flag
Human MYL2-4647H Recombinant Human MYL2 Protein (Ala2-Ile159), N-His tagged E.coli N-His Ala2-Ile159
Human MYL2-5258H Recombinant Human MYL2 protein, His-tagged E.coli His 8-164aa
Human MYL2-6759HF Recombinant Full Length Human MYL2 Protein, GST-tagged In Vitro Cell Free System GST 166 amino acids
Human MYL2-1467H-B Recombinant Human MYL2 Protein Pre-coupled Magnetic Beads HEK293
Mouse MYL2-10314M Recombinant Mouse Myl2 Protein, Myc/DDK-tagged HEK293T Myc/DDK
Mouse Myl2-4250M Recombinant Mouse Myl2 Protein, Myc/DDK-tagged HEK293T Myc/DDK
Rat MYL2-3854R Recombinant Rat MYL2 Protein Mammalian Cell His
Rat MYL2-3513R-B Recombinant Rat MYL2 Protein Pre-coupled Magnetic Beads HEK293
Rat MYL2-3513R Recombinant Rat MYL2 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Chicken MYL2-4680C Recombinant Chicken MYL2 Mammalian Cell His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • MYL2 Related Articles

What is MYL2 protein?

MYL2 (myosin light chain 2) gene is a protein coding gene which situated on the long arm of chromosome 12 at locus 12q24. Myosin light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. The MYL2 protein is consisted of 166 amino acids and its molecular mass is approximately 18.8 kDa.

What is the function of MYL2 protein?

MYL2 is a muscle-specific protein, its main function is to bind with myosin, form myosin complex, participate in myosin conformational change and intermyotome sliding, thereby regulating muscle contraction process. In addition, it plays an important role in muscle development and growth by participating in the proliferation and differentiation of muscle cells. It is also an ontractile protein that plays a role in heart development and function.

MYL2 Related Signaling Pathway

MYL2 can bind to calcium ions and participate in the regulation of calcium signaling pathways, regulating muscle contraction by binding Ca2+ ions and ATP. In addition, MYL2 can affect the occurrence and development of inflammatory response by regulating the expression and activity of inflammatory factors.

MYL2 Related Diseases

The mutated or mutated MYL2 gene is linked to a number of muscle and cardiovascular diseases, such as hypertrophic cardiomyopathy, congenital heart disease and arrhythmias. This suggests that MYL2 plays an important role in cardiac function and structural maintenance.

MYL2-7.jpg

Fig1. Hypertrophic cardiomyopathy (HCM) is caused by transcriptomic defects in certain proteins of the sarcomere as a result of specific genomic alterations on thick and thin filaments and Z-disks. (Marilena Melas, 2022)

Bioapplications of MYL2

In terms of scientific research applications, antibodies against MYL2 are widely used in a variety of scientific research fields, including Western Blot, immunocytochemistry, immunohistochemistry, ELISA and flow cytometry. In addition, researchers are conducting in-depth exploration of the relationship between MYL2 gene and myocardial diseases to explore therapeutic targets.

Case Study 1: Bo Zhao, 2023

MYL2-3.jpg

Fig1. Protein expression levels of Myh7, Atp1a3, Myl2, and Myl3 detected by western blotting.

Case Study 2: Pengcheng Pan, 2023

MYL2-4.jpg

Fig2. The plasmid was transfected into C2C12 cells. (a1–c1), cells were observed under a fluorescence microscope at 10×; (a2–c2), cells observed under an optical microscope at 10×. (a1,a2), cells transfected with pEGFP-N1; (b1,b2), cells transfected with pEGFP-N1-MYL2; (c1,c2), blank control group.

MYL2-5.jpg

Fig1. Overview of the mechanisms of cardiotoxicity caused by PS-MP. (Yue Zhou, 2023)

MYL2-6.jpg

Fig2. Schematic representation of the CaMKII-mediated signaling pathways that regulate expression of downstream genes during muscle adaptation to loading, and which are defective in C3KO muscles. (Jian Liu, 2020)

MYL2 involved in several pathways and played different roles in them. We selected most pathways MYL2 participated on our site, such as Cardiac muscle contraction, Adrenergic signaling in cardiomyocytes, Focal adhesion, which may be useful for your reference. Also, other proteins which involved in the same pathway with MYL2 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Cardiac muscle contractionCOX7A3;CACNA1SB;MYL3;TNNI3;CACNG2A;TPM4A;UQCRB;ATP1A1B;TMEM8C
Adrenergic signaling in cardiomyocytesVMHCL;GNAS;CACNA1D;ADCY1A;NETO2;ATP2B2;TNNT2;PPP2R2BB;GNAIA
Focal adhesionVCLA;MYL9;VWF;ITGA1;FYN;RHOAC;MYL12A;MAPK6;MAPK1
Tight junctionMPP5;EXOC4;SRC;PARD6B;ZAK;INADL;HCLS1;EPB41;ACTN3A
Leukocyte transendothelial migrationMAPK11;ROCK2;OCLN;NIPAL1;PRKCG;CLDN4;CLDN7;ITGAL;CTNNA3
Regulation of actin cytoskeletonPIP5K1C;RAC2;ABI2A;PIP5K2;PIK3R3A;ARHGEF7;ITGA6;FGF8A;VAV2
Hypertrophic cardiomyopathy (HCM)PRKAG3;TTN;CACNA2D1;LMNA;PRKAG1;ITGA3;DES;EMD;MYL3
Dilated cardiomyopathyTNF;TGFB2;CACNA2D2;PLN;DAG1;TTN;MYH7;LMNA;ITGA3

MYL2 has several biochemical functions, for example, actin monomer binding, calcium ion binding, myosin heavy chain binding. Some of the functions are cooperated with other proteins, some of the functions could acted by MYL2 itself. We selected most functions MYL2 had, and list some proteins which have the same functions with MYL2. You can find most of the proteins on our site.

Function Related Protein
actin monomer bindingTWF2;MYL4;ABL2;TMSB15B;ABL1;PKNOX2;COBLL1;MKL1B;NOS3
calcium ion bindingCALB2A;LRP2;PLS3;SYT17;Amy1;PCDH2G12;PCDH2AB2;STIM1;HPGDS
myosin heavy chain bindingMYL2;MYL9;PDLIM2;AMPD1;MYL12B;MYBPC3;CORO1A
protein bindingPSIP1;KATNBL1;CHD1;RNF185;RUNX3;CCDC28B;GNAI2;RAB35;THRAP3
structural constituent of muscleTTN;MYBPC3;MYL3;DAG1;PLEC;MYBPC2;ACTN2;ASPH;MYBPC1

MYL2 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with MYL2 here. Most of them are supplied by our site. Hope this information will be useful for your research of MYL2.

USP6; GJB5; LRSAM1; NHP2

Colson, BA; Petersen, KJ; et al. The myosin super-relaxed state is disrupted by estradiol deficiency. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 456:151-155(2015).
Wang, L; Muthu, P; et al. Diversity and similarity of motor function and cross-bridge kinetics in papillary muscles of transgenic mice carrying myosin regulatory light chain mutations D166V and R58Q. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY 62:153-163(2013).
  • Q&As
  • Reviews

Q&As (5)

Ask a question
Are there any gender or age-related differences in MYL2 protein expression in cardiac muscle? 07/21/2022

Studies suggest that MYL2 expression levels may vary with age and gender, which could have implications for understanding cardiac health across different demographics.

Can MYL2 be used as a prognostic marker for predicting outcomes in cardiac patients? 06/08/2022

Elevated MYL2 levels may be indicative of a poorer prognosis in certain cardiac conditions, providing valuable information for treatment planning.

What role does MYL2 play in personalized medicine for cardiac patients? 12/09/2017

Understanding the genetic variations in MYL2 allows for personalized treatment strategies tailored to an individual's specific cardiac profile.

How does MYL2 contribute to the understanding of the molecular mechanisms underlying cardiac hypertrophy? 07/10/2017

Dysregulation of MYL2 is implicated in hypertrophic cardiomyopathy, contributing to our understanding of the molecular pathways leading to cardiac hypertrophy.

How can MYL2 protein analysis aid in risk stratification for cardiovascular diseases? 03/31/2017

Analyzing MYL2 variants and expression levels can help identify individuals at a higher risk of developing cardiac disorders, allowing for proactive management.

Customer Reviews (3)

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Reviews
04/09/2017

    The MYL2 Protein comes highly recommended for its exceptional performance, particularly in ELISA assays.

    11/02/2016

      This protein has proven to be of great utility in protein electron microscopy structure analysis.

      09/19/2016

        Researchers utilizing the MYL2 Protein can rely on its robust and reproducible performance in a variety of experimental settings.

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