NAMPT

Case study 1: Qianyang Liu, 2023

Nicotinamide phosphoribosyl transferase (NAMPT) is associated with various NAD+ -consuming enzymatic reactions. The precise role in intestinal mucosal immunity in necrotizing enterocolitis (NEC) is not well defined. Here, the researchers examined whether NAMPT inhibition by the highly specific inhibitor FK866 could alleviate intestinal inflammation during the pathogenesis of NEC.

NAMPT expression was upregulated in the human terminal ileum of human infants with NEC. FK866 inhibited intercellular NAD+ levels, macrophage M1 polarization, and the expression of NAD+ -dependent enzymes. Consistently, the capacity of macrophages to phagocytose zymosan particles, as well as antibacterial activity, were impaired by FK866, whereas NMN supplementation to restore NAD+ levels reversed the changes in phagocytosis and antibacterial activity.

Fig1. Western blot analysis was performed to examine NAMPT expression in ileal homogenates from human patients and controls.

Fig2. NAD+ concentrations were determined in the terminal ileum of animals that were treated as indicated (n = 6).

Case study 2: Justyna Gogola-Mruk, 2023

Ovarian cancer is characterized by recurrent peritoneal and distant metastasis. To survive in a non-adherent state, floating ovarian cancer spheroids develop mechanisms to resist anoikis. However, the mechanism by which visfatin induces anoikis resistance in ovarian cancer spheroids remains unknown. Here, the researchers aimed to assess wheather visfatin which possess anti-apoptotic properties can induce resistance of anoikis in ovarian cancer spheroids. Visfatin synthesis were examined using a commercial human visfatin ELISA Kit. Spheroid were exposed to visfatin and cell viability and caspase 3/7 activity were measured using CellTiter-Glo 3D cell viability assay and Caspase-Glo® 3/7 Assay System. mRNA and protein expression were analyzed by Real-time PCR and Western Blot analysis, respectively. higher expression and secretion of visfatin by epithelial than by granulosa ovarian cells, and in non-cancer tissues versus cancer tissues. Interestingly, visfatin increased the proliferation/apoptosis ratio in ovarian cancer spheroids. Specifically, both the intrinsic and extrinsic pathways of anoikis were regulated by visfatin.

Fig3. Effect of visfatin on the proliferation/apoptosis ratio in OVCAR-3, SKOV-3 and KGN cells.

Fig4. Dose-dependent effect of visfatin (10, 100 and 1000 ng/mL) on caspase-3 activity in KGN.

Fig1. PBEF stimulates expression and release of other cytokines. (Zhongjie Sun, 2013)

Fig2. NAMPT and the regulation of inflammation. (E S Burgos, 2011)

NAMPT involved in several pathways and played different roles in them. We selected most pathways NAMPT participated on our site, such as Adipogenesis, BMAL1:CLOCK,NPAS2 activates circadian gene expression, Circadian Clock, which may be useful for your reference. Also, other proteins which involved in the same pathway with NAMPT were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

NAMPT has several biochemical functions, for example, cytokine activity, drug binding, nicotinamide phosphoribosyltransferase activity. Some of the functions are cooperated with other proteins, some of the functions could acted by NAMPT itself. We selected most functions NAMPT had, and list some proteins which have the same functions with NAMPT. You can find most of the proteins on our site.

NAMPT has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with NAMPT here. Most of them are supplied by our site. Hope this information will be useful for your research of NAMPT.

FTL; MT-ND1; IFITM3; DDA1; UBE2L6; GGT1; ADORA2A; AKT2; sseA; dcyD