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PRPF8

Official Full Name

pre-mRNA processing factor 8
Overview

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq, Jul 2008]
Synonyms

PRPF8; pre-mRNA processing factor 8; PRP8; RP13; HPRP8; PRPC8; SNRNP220; pre-mRNA-processing-splicing factor 8; p220; PRP8 homolog; splicing factor Prp8; apoptosis-regulated protein 1; apoptosis-regulated protein 2; 220 kDa U5 snRNP-specific protein; precursor mRNA processing protein; PRP8 pre-mRNA processing factor 8 homolog; U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p;

Species	Cat.#	Product name	Source (Host)	Tag	Protein Length	Price
Human	PRPF8-1979H	Recombinant Human PRPF8, GST-tagged	E.coli	GST	C-term-300aa	$319 / 25ug
Zebrafish	PRPF8-11211Z	Recombinant Zebrafish PRPF8	Mammalian Cell	His		Inquiry

Involved Pathway
Protein Function
Interacting Protein

PRPF8 involved in several pathways and played different roles in them. We selected most pathways PRPF8 participated on our site, such as Gene Expression, Processing of Capped Intron-Containing Pre-mRNA, Spliceosome, which may be useful for your reference. Also, other proteins which involved in the same pathway with PRPF8 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name	Pathway Related Protein
Gene Expression	GTF3C5;IGF2BP3;ZNF565;ZNF599;ZNF571;COBRA1;ZNF777;ZFP473;NR2E3
Processing of Capped Intron-Containing Pre-mRNA	HNRNPA2B1;SF3B4;SNRNP25;PAPOLA;PDCD7;SNRNP48;CD2BP2;THOC4;TTC6
Spliceosome	CTNNBL1;SF3B4;DHX15;SNRNP27;HNRNPK;DHX8;SNRPG;SRSF7;CHERP
mRNA Splicing	PRPF8;PTBP1B;HNRNPA0;RNPC3;HNRNPL2;HNRNPF;HNRNPH1;PDCD7;TTC6
mRNA Splicing - Major Pathway	PTBP1;HNRNPH1;HNRNPL2;SF3A3;RBM5;DNAJC8;HNRNPR;YBX1;SUGP1
mRNA Splicing - Minor Pathway	ZMAT5;SNRNP35;SF3B4;PDCD7;SNRNP25;RNPC3;SF3B2;PRPF8;YBX1
mRNA processing	FAM120B;BICC1;HNRNPR;CD2BP2;ADARB2;SPOP;PPM1G;HNRNPD;SF3A1

PRPF8 has several biochemical functions, for example, K63-linked polyubiquitin binding, U1 snRNA binding, U2 snRNA binding. Some of the functions are cooperated with other proteins, some of the functions could acted by PRPF8 itself. We selected most functions PRPF8 had, and list some proteins which have the same functions with PRPF8. You can find most of the proteins on our site.

Function	Related Protein
K63-linked polyubiquitin binding	UIMC1;RNF168;ATRIP;SPRTN;IKBKG;ZRANB1;ZRANB3;PRPF8;ZRANB1B
U1 snRNA binding	ISG20;SNRPA;SNRNP70;PRPF8
U2 snRNA binding	SNRPA1;PRPF8;ISG20;TROVE2
U5 snRNA binding
U6 snRNA binding	SART3;DDX39B;LSM2;EIF5A;LSM7;PRPF4;LSM8;PRPF8;RBM22
poly(A) RNA binding	UBE2I;DHX36;ACTN4;PEF1;KHDRBS3;SLC16A3;RBM19;TRMT2A;RNPS1
pre-mRNA intronic binding	SOX9;RBM4;RNPC3;RBM41;EP300;HNRNPA2B1;PRPF8
protein binding	BBS7;GNL3;DYNLL2;DCTPP1;RAD50;SPATA7;ALOX12;HPCAL1;SOD1
second spliceosomal transesterification activity	SLU7;PRPF8

PRPF8 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with PRPF8 here. Most of them are supplied by our site. Hope this information will be useful for your research of PRPF8.

SNRNP40; PRPF4; Snw1; SNW1; EFTUD2; BCAS2; SLU7; SF3B2; PCBP1