RSPO1
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Official Full Name
R-spondin 1
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Overview
This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] -
Synonyms
RSPO1; R-spondin 1; RSPO; CRISTIN3; R-spondin-1; R-spondin homolog; roof plate-specific spondin-1;
- Recombinant Proteins
- GMP Proteins
- Cell & Tissue Lysates
- Human
- Mouse
- Rat
- Zebrafish
- CHO
- CHO-K1
- E.coli
- HEK293
- Human Cell
- Mammalian Cell
- Mammalian cells
- C
- His
- Fc
- His(C
- ter)
- His|T7
- N/A
- N
- SUMO
- Tag Free
- Involved Pathway
- Protein Function
- Interacting Protein
- RSPO1 Related Research Area
- RSPO1 Related Signal Pathway
RSPO1 involved in several pathways and played different roles in them. We selected most pathways RSPO1 participated on our site, such as Regulation of FZD by ubiquitination, Signal Transduction, Signaling by Wnt, which may be useful for your reference. Also, other proteins which involved in the same pathway with RSPO1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| Regulation of FZD by ubiquitination | LGR4;RSPO4;LGR5;FMNL3;ZNRF3;RSPO3;RNF43;LGR6;RSPO2 |
| Signal Transduction | CCNT2A;VIP2;GPR39;MKS1;FMNL2;TLE4;PROKR1A;PENKB;AMOTL2B |
| Signaling by Wnt | SOX9A;RBBP5;SOX32;ASH2L;ROR1;KREMEN2;TMED5;SOX6;RYK |
| TCF dependent signaling in response to WNT | SRY;ASH2L;WNT8-2;WIF1;CDC73;PYGO1;TLE3B;RSPO2;SOX9B |
| Wnt signaling network | ATP6AP2;RSPO1;IGFBP4;WIF1;CTHRC1;RYK |
RSPO1 has several biochemical functions, for example, G-protein coupled receptor binding, heparin binding, protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by RSPO1 itself. We selected most functions RSPO1 had, and list some proteins which have the same functions with RSPO1. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| G-protein coupled receptor binding | UCN3L;APLN;HSPA8;RSPO4;QRFP;RSPO1;GNAL;ADORA1;GHRL |
| heparin binding | PRG2;REG4;PLA2G5;C6orf25;PDCD5;APPA;LIPI;APP;COL20A1 |
| protein binding | PAM16;GRM1;SAFB2;TAF1B;FCNA;ERC1;PKIA;DRG2;NFATC1 |
| receptor binding | NPFF;NRG4;TMED1;FASLG;CADM1A;LBP;ADM;FGF20A;MBL2 |
RSPO1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with RSPO1 here. Most of them are supplied by our site. Hope this information will be useful for your research of RSPO1.
ZNRF3
- Q&As
- Reviews
Q&As (7)
Ask a questionRSPO1 stands out for its potential therapeutic use in Regenerative Medicine due to its mitogenic activity in stem cells.
Characterization methods included Western blotting, mass spectrometry, and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming structural integrity and biological efficacy.
RSPOs regulate various tissue-specific processes, including bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells, intestinal stem cells, and cancer progression.
The purification strategy for rhRSPO1 involved two sequential chromatographic steps: heparin affinity chromatography followed by molecular exclusion chromatography.
RSPO1 induces the Wnt pathway by stabilizing the Wnt receptor complex, leading to enhanced Wnt signaling and influencing cellular processes.
rhRSPO1 was generated using the HEK293 cell line, and the coding sequence was synthesized and subcloned into a mammalian cell expression vector.
R-Spondin proteins, including RSPO1, play crucial roles in cell proliferation, differentiation, and death by inducing the Wnt pathway.
Customer Reviews (3)
Write a reviewRemarkable changes in cell behavior were attributed to the potent effects of the product.
The product's respected reputation in the scientific community was a testament to its quality and effectiveness.
Dependable protein-protein interaction outcomes were achieved due to the product's pure composition.
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