"TR" Related Products

        TR (human) Reporter Assay Kit

        Cat.No. : Kit-2035
        Size : 2 x 48 panel
        Description : The panel of TR reporter assays utilizes non-human mammalian cells engineered to express human thyroid hormone receptors alpha (NR1A1) and beta (NR1A2), ligand-dependent transcription factors commonly referred to as TRα and TRβ. TheTR reporter cells include the luciferase reporter gene functionally linked to a responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in TRα or TRβ activity. The principal application of this assay panel is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against the human TR"s. TR reporter cells are prepared using the proprietary CryoMite process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the pre-incubation of reporter cells prior to assay setup. The nuclear receptor reporter assays are all-inclusive cell-based assay systems. In addition to TR reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user"s test samples, a reference agonist, luciferase detection reagent, and a cell culture-ready assay plate.
        Storage : -80°C
        Kit Components : TRα Reporter Cells: 1 x 1.0 mL; -80°C▪ TRβ Reporter Cells: 1 x 1.0 mL; -80°C ▪ Cell Recovery Media (CRM): 1 x 10.5 mL; -20°C▪ Compound Screening Media (CSM): 1 x 35 mL; -20°C ▪ L-Triiodothyronine, 30 mM (in DMSO) (reference agonist for TRα and TRβ): 1 x 30 µL; -20°C▪ Detection Substrate: 2 x 3.0 mL; -80°C▪ Detection Buffer: 2 x 3.0 mL; -20°C▪ Plate frame: 1; ambient ▪ snap-in, 8-well strips: 12; ambient

        Online Inquiry

        Note: There will be extra charge for optional service!

        Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

        Optional requirements on this protein    +Expand

        Price Inquiry

        Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.