Nature Method has recently sorted out the most noteworthy technologies in 2016, including protein labeling in cells, unraveling nuclear architecture, protein structure though time, precision optogenetics, highly multiplexed imaging, deep learning, subcellular maps and integrated single-cell profiles.   Florescent dyes are relatively small, having good photophysical properties and spectral span. Those features make fluorescent dyes attractive, and they are expected to replace fluorescent protein for protein labeling. Researchers are actively…