RAS participates in multiple signaling pathways, and post-translational modifications regulate the subcellular localization of RAS to regulate downstream signal transduction. Among them, palmitoylation is a necessary modification of HRAS and NRAS signal transduction. The acyltransferase DHHC9 binds to the helper protein GCP16 to catalyze palmitoylation of HRAS and NRAS, but the interaction between GCP16 and DHHC9, as well as the molecular mechanism by which GCP16 regulates the palmitoylation activity of…