2-Deoxyglucose Colorimetric Assay Kit

Cat.No. :
Kit-0003
Product Overview :
2-Deoxyglucose Assay Kit (Colorimetric) is a quantitative colormetric determination of 2-deoxyglucose at 412 nm.
Description :
Glucose uptake is an important biological tool for studying cell signaling and glucose metabolism. Of many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been most widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells.In 2-Deoxyglucose Assay Kit (Colorimetric), 2-DG6P is oxidized to generate NADPH, which is determined by an enzymatic recycling amplification reaction. This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/sample.
Applications :
Measurement of glucose uptake in response to insulin, growth factors, cytokines, mitogens, and nutrients, etc. Analysis of glucose metabolism and cell signaling in various cell types. Screening anti-diabetic drugs.
Target Species :
Mammals
Usage :
For research use only (RUO)
Storage :
Store kit at -20°C, protected from light. Warm all Buffers to room temperature before use. Briefly centrifuge all small vials prior to opening.
Kit Components :
Extraction Buffer. Cap code: NM. 17 mlNeutralization Buffer. Cap code: clear. 2.5 ml2-Deoxyglucose (2-DG, 10 mM). Cap code: purple. 1 mlAssay Buffer. Cap code: WM. 10 mlEnzyme Mix (Lyophilized). Cap code: orange. 1 vialRecycling Mix (Lyophilized). Cap code: blue. 1 vial2-DG6P Standard (Lyophilized). Cap code: yellow. 1 vialGlutathione Reductase (Lyophilized). Cap code: green. 2 vialsSubstrate-DTNB (Lyophilized). Cap code: red. 2 vials
Detection method :
Colorimetric
Compatible Sample Types :
Attached Cell, Suspension Cell
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry

We are here to answer any question you may have