Product Overview :
2-Deoxyglucose Assay Kit (Fluorometric) is a quantitative fluorometric determination of 2-deoxyglucose.
Description :
Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is, therefore, directly proportional to 2-DG (or glucose) uptake by cells. In 2-Deoxyglucose Assay Kit (Fluorometric), the accumulated 2-DG6P is enzymatically oxidized and coupled to PicoProbe, which generates fluorescence in the presence of NADPH. This easy to use non-radioactive kit can detect glucose uptake as low as 50 pmol/well and can be used for a variety of cell types.
Applications :
Measurement of glucose uptake in response to insulin, growth factors, cytokines, mitogens and nutrients, etc. Analysis of glucose metabolism and cell signaling in various cell types. Screening anti-diabetic drugs.
Usage :
For research use only (RUO)
Storage :
Store kit at -20°C, protected from light. Warm all Buffers to room temperature before use. Briefly centrifuge all small vials prior to opening.
Kit Components :
Extraction Buffer. Cap code: NM. 17 mlNeutralization Buffer. Cap code: clear. 1 ml2-Deoxyglucose (2-DG, 10 mM). Cap code: purple. 1 ml2-DG Uptake Assay Buffer. Cap code: MW. 10 mlEnzyme Mix (Lyophilized). Cap code: green. 1 VialPicoProbeTM (in DMSO). Cap code: blue. 0.2 ml2-DG6P Standard (Lyophilized). Cap code: yellow. 1 Vial
Detection method :
Fluorometric
Compatible Sample Types :
Cell Lysate
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