Product Overview :
5-Lipoxygenase (5-LOX) is a non-heme iron-containing dioxygenase that converts unsaturated fatty acids to epoxides; for example the synthesis of leukotrienes from arachidonic acid. It is involved in processes like cell proliferation, differentiation and inflammation and has been implicated in inflammation and hyper proliferation mediated diseases like asthma, rheumatoid arthritis and cancer. Research for identifying potential remedies for such diseases involves the identification of inhibitors of 5-lipoxygenase. 5-Lipoxygenase Inhibitor Screening Kit uses Zileuton, a well-known 5-lipoxygenase inhibitor as positive control, and provides a simple and fast method to screen/ characterize potential 5-lipoxygenase inhibitors.
Storage :
Upon arrival, store the kit at -80°C, protected from light. Briefly centrifuge small vials before opening. Read entire protocol before performing the assay. Components are stable for at least three months.
• LOX Assay Buffer: Warm to room temperature before use.
• LOX Probe and Zileuton: Aliquot and store at -80ºC in the dark.
• LOX Substrate: Store at -80ºC.
5-LOX Enzyme: Aliquot and store at -80ºC. Avoid repeated freeze thaw cycles. Note: Keep all components on ice while performing the assay.
Kit Components :
LOX Assay Buffer 25 ml
LOX Probe 200 µl
LOX Substrate 6 µl
Zileuton 100 µl
5-LOX Enzyme 300 U
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