Product Overview :
Phosphate concentrations between 1 µM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined. (500 assays) The assay detects acid phosphatase (AP) activity conveniently in various biological samples.
Description :
Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease. Acid Phosphatase Assay Kit is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The Acid Phosphatase Activity kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax = 405 nm) when dephosphorylated by AP. The kit can detect µU acid phosphatase activity in samples.
Applications :
Phosphate concentrations between 1 µM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined.
Target Species :
Mammalian
Usage :
This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
Kit Components :
AP Assay BufferpNPP Substrate (10 TAB)AP EnzymeStop Solution
Detection method :
Colorimetric
Compatible Sample Types :
Cell Culture SupernatantsPlasmaSerumTissue LysatesUrineCell Lysates
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