Product Overview :
Alkaline Phosphatase Assay Kit is a colorimetric kinetic determination of serum alkaline phosphatase activity.
Description :
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis.
Applications :
Direct Assays: ALP activity in serum, plasma and other sources.Characterization and Quality Control for ALP production.Drug Discovery: high-throughput screen for ALP inhibitors and evaluation of ALP inhibitors.
Usage :
For research use only (RUO)
Storage :
The kit is shipped at room temperature. Store pNPP Liquid at -20°C and other components at 4°C. Shelf life of 12 months after receipt.
Kit Components :
Assay Buffer 50 ml, pH 10.5Mg Acetate 1.5 mL, 0.2 MpNPP Liquid 600 µL, 1 MCalibrator 10 mL, Tartrazine
Detection method :
Colorimetric
Compatible Sample Types :
Plasma, Serum
Features & Benefits :
High sensitivity and wide linear range: Use 5 µL serum or plasma sample. The detection limit is 2 U/L, linear up to 800 U/L.Homogeneous and simple procedure: Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 5 minutes.Robust and amenable to HTS: All reagents are compatible with high-throughput liquid handling instruments.
PDF