The assay is initiated with the enzymatic hydrolysis of the glucoside by α-Galactosidase. The enzyme catalysed reaction products p-nitrophenol, can be measured at a colorimetric readout at 400 nm.
Description :
Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It is encoded by the GLA gene. Two recombinant forms of alpha-galactosidase are called agalsidase alfa (INN) and agalsidase beta (INN). This enzyme is a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose.
96-Well Microplate: 1 plate Assay Buffer: 100 ml x 1; 4 °C Reaction Buffer: 4 ml x 1; 4 °C Substrate: Powder x 1; -20 °C Stop Solution: 15 ml x 1; 4 °C Standard (5μmol/mL p-nitrophenol solution): 1 ml x 1; 4 °C Note: Substrate: For each tube, add 2.5 ml distilled water to dissolve before use.
Target Species :
Detection and Quantification of Alpha-Galactosidase (α-GAL) Activity in Tissue extracts, Cell lysate, Cell culture media and Other biological fluids Samples.
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