Common Name :
Asparaginase
Product Overview :
Simple procedure; takes 40 minutes
Fast, reliable and convenient
Kit can be used to detect Asparaginase enzyme's antineoplastic effects, based on the inability of cancer cells (unlike healthy cells) to synthesize asparagine.
Description :
Asparaginase (EC 3.5.1.1) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs). The enzyme has been used to reduce acrylamide, a suspected carcinogen, produced in fried starchy food products and to treat acute lymphoblastic leukemia (ALL) and some other hematopoietic neoplasms (e.g. multiple myeloma). Metabolization of asparagine prevents acrylamide formation in fried foods (Maillard reaction). The enzyme's antineoplastic effects are based on the inability of cancer cells (unlike healthy cells) to synthesize asparagine. However, the enzyme is not without some antigenicity and toxicity so it is very important to measure its activity in biological samples or monitor its activity during therapy. We provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples. In the assay, Asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected colorimetrically (λ = 570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction.
Usage :
This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
Kit Components :
Asparaginase Assay Buffer
OxiRed Probe (in DMSO)
Substrate Mix
Aspartate Enzyme Mix
Conversion Mix
Positive Control
Aspartate Standard (100 mM)
Detection method :
Colorimetric
Fluorescence Plate Reader
Compatible Sample Types :
Bacteria
Food Products
Tissue Lysates
Cell Lysates
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